View source for 8rfk


==Soluble glucose dehydrogenase from acinetobacter calcoaceticus - single mutant pH8==
<StructureSection load='8rfk' size='340' side='right'caption='[[8rfk]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8rfk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_calcoaceticus Acinetobacter calcoaceticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RFK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1H0D:3-(3,5-dicarboxy-1~{H}-pyrrol-2-yl)pyridine-2,4,6-tricarboxylic+acid'>A1H0D</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rfk OCA], [https://pdbe.org/8rfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rfk RCSB], [https://www.ebi.ac.uk/pdbsum/8rfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rfk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DHGB_ACICA DHGB_ACICA] Oxidizes glucose to gluconolactone.
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== Publication Abstract from PubMed ==
The soluble glucose dehydrogenase (sGDH) from Acinetobacter calcoaceticus has been widely studied and is used, in biosensors, to detect the presence of glucose, taking advantage of its high turnover and insensitivity to molecular oxygen. This approach, however, presents two drawbacks: the enzyme has broad substrate specificity (leading to imprecise blood glucose measurements) and shows instability over time (inferior to other oxidizing glucose enzymes). We report the characterization of two sGDH mutants: the single mutant Y343F, and the double mutant D143E/Y343F. The mutants present enzyme selectivity and specificity of 1.2 (Y343F) and 5.7 (D143E/Y343F) times higher for glucose compared to that of the wild type. Crystallographic experiments, designed to characterise these mutants, surprisingly revealed that the prosthetic group PQQ (pyrroloquinoline quinone), essential for the enzymatic activity, is in a cleaved form for both wild-type and mutant structures. We provide evidence suggesting that the sGDH produces H2O2, the level of production depending on the mutation. In addition, spectroscopic experiments allowed us to follow the self-degradation of the prosthetic group and the disappearance of sGDH's glucose oxidation activity. These studies suggest that the enzyme is sensitive to its self-production of H2O2. We show that the premature aging of sGDH can be slowed down by adding catalase to consume the H2O2 produced, allowing the design of a more stable biosensor over time. Our research opens questions about the mechanism of H2O2 production and the physiological role of this activity by sGDH.

Does Acinetobacter calcoaceticus glucose dehydrogenase produce self-damaging H2O2?,Lublin V, Kauffmann B, Engilberge S, Durola F, Gounel S, Bichon S, Jean C, Mano N, Giraud MF, Chavas L, Thureau A, Thompson A, Stines-Chaumeil C Biosci Rep. 2024 Apr 30:BSR20240102. doi: 10.1042/BSR20240102. PMID:38687614<ref>PMID:38687614</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8rfk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Acinetobacter calcoaceticus]]
[[Category: Large Structures]]
[[Category: Chavas L]]
[[Category: Giraud MF]]
[[Category: Kauffmann B]]
[[Category: Lublin V]]
[[Category: Stines-Chaumeil C]]
[[Category: Thompson A]]