View source for 8rj0


==Crystal structure of mutant aspartase from Bacillus sp. YM55-1 in the closed loop conformation==
<StructureSection load='8rj0' size='340' side='right'caption='[[8rj0]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8rj0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._YM55-1 Bacillus sp. YM55-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RJ0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rj0 OCA], [https://pdbe.org/8rj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rj0 RCSB], [https://www.ebi.ac.uk/pdbsum/8rj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rj0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ASPA_BACSP ASPA_BACSP] Catalyzes the reversible conversion of L-aspartate to fumarate and ammonia (PubMed:10334861, PubMed:10712618, PubMed:18844200, PubMed:19490103). Is highly specific for L-aspartate in the deamination reaction, and cannot use alternative substrates such as D-aspartic acid, alpha-methyl-DL-aspartic acid, beta-methyl-DL-aspartic acid or L-glutamate (PubMed:18844200). In the reverse reaction, alternative nucleophiles (such as hydroxylamine, hydrazine, methoxylamine and methylamine) can replace ammonia in vitro, leading to the formation of N-substituted aspartic acid derivatives (PubMed:18844200).<ref>PMID:10334861</ref> <ref>PMID:10712618</ref> <ref>PMID:18844200</ref> <ref>PMID:19490103</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus sp. YM55-1]]
[[Category: Large Structures]]
[[Category: Capra N]]
[[Category: Janssen DB]]
[[Category: Thunnissen AMWH]]