View source for 8rjw


==Human RAD52 open ring - ssDNA complex==
<StructureSection load='8rjw' size='340' side='right'caption='[[8rjw]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8rjw]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RJW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rjw OCA], [https://pdbe.org/8rjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rjw RCSB], [https://www.ebi.ac.uk/pdbsum/8rjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rjw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RAD52_HUMAN RAD52_HUMAN] Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.<ref>PMID:12379650</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
RAD52 is important for the repair of DNA double-stranded breaks(1,2), mitotic DNA synthesis(3-5) and alternative telomere length maintenance(6,7). Central to these functions, RAD52 promotes the annealing of complementary single-stranded DNA (ssDNA)(8,9) and provides an alternative to BRCA2/RAD51-dependent homologous recombination repair(10). Inactivation of RAD52 in homologous-recombination-deficient BRCA1- or BRCA2-defective cells is synthetically lethal(11,12), and aberrant expression of RAD52 is associated with poor cancer prognosis(13,14). As a consequence, RAD52 is an attractive therapeutic target against homologous-recombination-deficient breast, ovarian and prostate cancers(15-17). Here we describe the structure of RAD52 and define the mechanism of annealing. As reported previously(18-20), RAD52 forms undecameric (11-subunit) ring structures, but these rings do not represent the active form of the enzyme. Instead, cryo-electron microscopy and biochemical analyses revealed that ssDNA annealing is driven by RAD52 open rings in association with replication protein-A (RPA). Atomic models of the RAD52-ssDNA complex show that ssDNA sits in a positively charged channel around the ring. Annealing is driven by the RAD52 N-terminal domains, whereas the C-terminal regions modulate the open-ring conformation and RPA interaction. RPA associates with RAD52 at the site of ring opening with critical interactions occurring between the RPA-interacting domain of RAD52 and the winged helix domain of RPA2. Our studies provide structural snapshots throughout the annealing process and define the molecular mechanism of ssDNA annealing by the RAD52-RPA complex.

Mechanism of single-stranded DNA annealing by RAD52-RPA complex.,Liang CC, Greenhough LA, Masino L, Maslen S, Bajrami I, Tuppi M, Skehel M, Taylor IA, West SC Nature. 2024 Apr 24. doi: 10.1038/s41586-024-07347-7. PMID:38658755<ref>PMID:38658755</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8rjw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Liang CC]]
[[Category: West SC]]