View source for 8rvj


==Engineered Encapsulin P3P4==
<StructureSection load='8rvj' size='340' side='right'caption='[[8rvj]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8rvj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Quasibacillus_thermotolerans Quasibacillus thermotolerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RVJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rvj OCA], [https://pdbe.org/8rvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rvj RCSB], [https://www.ebi.ac.uk/pdbsum/8rvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rvj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ENCAP_BACTR ENCAP_BACTR] Shell component of a type 1 encapsulin nanocompartment. Assembles into proteinaceous icosahedral shells 42-43 nm in diameter with an iron- and phosphorus-rich core (1Fe:1.1P) which can store over 23,000-35,000 iron atoms (with a calculated maximum of 83,000 Fe). There are 2 types of negatively charged open pores in the cryo-electron structure; a 3-fold pore where 3 hexamers meet with a minimal size of 7.2 Angstroms and a 5-fold pore where pentamers meet with a minimal size of 2.3 Angstroms. The 2-fold pore seen in other encapsulin nanocompartments is closed. Empty compartments can be generated in E.coli (PubMed:28263314, PubMed:31282860, PubMed:31194509). Both types of pore have extra density in their centers in the structure (PubMed:31282860). 2 different cargo proteins have been identified (IMEF and Fer); when both are expressed in E.coli with the shell protein only IMEF is detected within the nanocompartment. E.coli expressing all 3 genes stores the largest amount of iron and is protected from Fe/H2O2-induced oxidative stress (PubMed:28263314). Part of the iron-mineralizing encapsulin-associated Firmicute (IMEF) system (Probable).<ref>PMID:28263314</ref> <ref>PMID:31194509</ref> <ref>PMID:31282860</ref> <ref>PMID:28263314</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Quasibacillus thermotolerans]]
[[Category: Jerala R]]
[[Category: Satler T]]