View source for 8rvr


==Crystal structure of Trypanosoma congolense pyruvate kinase in complex with a single-domain antibody (TcoPYK-sdAb42) in the presence of sulfate==
<StructureSection load='8rvr' size='340' side='right'caption='[[8rvr]], [[Resolution|resolution]] 3.19&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8rvr]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_congolense Trypanosoma congolense] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RVR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.19&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rvr OCA], [https://pdbe.org/8rvr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rvr RCSB], [https://www.ebi.ac.uk/pdbsum/8rvr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rvr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G0UYF4_TRYCI G0UYF4_TRYCI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
African trypanosomes are the causative agents of neglected tropical diseases affecting both humans and livestock. Disease control is highly challenging due to an increasing number of drug treatment failures. African trypanosomes are extracellular, blood-borne parasites that mainly rely on glycolysis for their energy metabolism within the mammalian host. Trypanosomal glycolytic enzymes are therefore of interest for the development of trypanocidal drugs. Here, we report the serendipitous discovery of a camelid single-domain antibody (sdAb aka Nanobody) that selectively inhibits the enzymatic activity of trypanosomatid (but not host) pyruvate kinases through an allosteric mechanism. By combining enzyme kinetics, biophysics, structural biology, and transgenic parasite survival assays, we provide a proof-of-principle that the sdAb-mediated enzyme inhibition negatively impacts parasite fitness and growth.

Allosteric inhibition of trypanosomatid pyruvate kinases by a camelid single-domain antibody.,Pinto Torres JE, Claes M, Hendrickx R, Yuan M, Smiejkowska N, Van Wielendaele P, Hacisuleyman A, De Winter H, Muyldermans S, Michels PAM, Walkinshaw MD, Versees W, Caljon G, Magez S, Sterckx YG Elife. 2025 Mar 31;13:RP100066. doi: 10.7554/eLife.100066. PMID:40163365<ref>PMID:40163365</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8rvr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Trypanosoma congolense]]
[[Category: Vicugna pacos]]
[[Category: Sterckx YG-J]]