View source for 8rza


==Ribonuclease W==
<StructureSection load='8rza' size='340' side='right'caption='[[8rza]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8rza]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RZA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rza OCA], [https://pdbe.org/8rza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rza RCSB], [https://www.ebi.ac.uk/pdbsum/8rza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rza ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FAU1_PYRFU FAU1_PYRFU] Probable RNase involved in rRNA stability through maturation and/or degradation of precursor rRNAs. Preferentially cleaves UA sequences in the 5' precursor region of 5S rRNA (PubMed:28978920). Binds to RNA in loop regions with AU-rich sequences. Binds to the consensus sequence GGC(U/A)(U/A)U(U/C) in vitro (PubMed:12614195).<ref>PMID:12614195</ref> <ref>PMID:28978920</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ribosome biogenesis is a complex process requiring multiple precursor ribosomal RNA (rRNA) cleavage steps. In archaea, the full set of ribonucleases (RNases) involved in rRNA processing remains to be discovered. A previous study suggested that FAU-1, a conserved protein containing an RNase G/E-like protein domain fused to a domain of unknown function (DUF402), acts as an RNase in archaea. However, the molecular basis of this activity remained so far elusive. Here, we report two X-ray crystallographic structures of RNase G/E-like-DUF402 hybrid proteins from Pyrococcus furiosus and Sulfolobus acidocaldarius, at 2.1 and 2.0 A, respectively. The structures highlight a structural homology with the 5' RNA recognition domain of Escherichia coli RNase E but no homology with other known catalytic nuclease domains. Surprisingly, we demonstrate that the C-terminal domain of this hybrid protein, annotated as a putative diphosphatase domain, harbors the RNase activity. Our functional analysis also supports a model by which the RNase G/E-like domain acts as a regulatory subunit of the RNase activity. Finally, in vivo experiments in Haloferax volcanii suggest that this RNase participates in the maturation of pre-16S rRNA. Together, our study defines a new RNase family, which we termed the RNase W family, as the first archaea-specific contributor to archaeal ribosome biogenesis.

RNase W, a conserved ribonuclease family with a novel active site.,Vayssieres M, Juttner M, Haas K, Ancelin A, Marchfelder A, Leulliot N, Ferreira-Cerca S, Blaud M Nucleic Acids Res. 2024 Oct 24:gkae907. doi: 10.1093/nar/gkae907. PMID:39445822<ref>PMID:39445822</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8rza" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Pyrococcus furiosus]]
[[Category: Blaud M]]
[[Category: Leulliot N]]
[[Category: Vayssieres M]]