View source for 8sp9


==Crystal Structure of Coxsackievirus B3 (CVB3) Cloverleaf RNA with tRNA scaffold==
<StructureSection load='8sp9' size='340' side='right'caption='[[8sp9]], [[Resolution|resolution]] 2.92&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8sp9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Coxsackievirus_B3 Coxsackievirus B3] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SP9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.92&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8sp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8sp9 OCA], [https://pdbe.org/8sp9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8sp9 RCSB], [https://www.ebi.ac.uk/pdbsum/8sp9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8sp9 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The genomes of positive-strand RNA viruses serve as a template for both protein translation and genome replication. In enteroviruses, a cloverleaf RNA structure at the 5' end of the genome functions as a switch to transition from viral translation to replication by interacting with host poly(C)-binding protein 2 (PCBP2) and the viral 3CDpro protein. We determined the structures of cloverleaf RNA from coxsackievirus and poliovirus. Cloverleaf RNA folds into an H-type four-way junction and is stabilized by a unique adenosine-cytidine-uridine (A*C-U) base triple involving the conserved pyrimidine mismatch region. The two PCBP2 binding sites are spatially proximal and are located on the opposite end from the 3CDpro binding site on cloverleaf. We determined that the A*C-U base triple restricts the flexibility of the cloverleaf stem-loops resulting in partial occlusion of the PCBP2 binding site, and elimination of the A*C-U base triple increases the binding affinity of PCBP2 to the cloverleaf RNA. Based on the cloverleaf structures and biophysical assays, we propose a new mechanistic model by which enteroviruses use the cloverleaf structure as a molecular switch to transition from viral protein translation to genome replication.

Structural basis for cloverleaf RNA-initiated viral genome replication.,Gottipati K, McNeme SC, Tipo J, White MA, Choi KH Nucleic Acids Res. 2023 Jul 24:gkad618. doi: 10.1093/nar/gkad618. PMID:37486760<ref>PMID:37486760</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 8sp9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Coxsackievirus B3]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Choi KH]]
[[Category: Gottipati K]]
[[Category: McNeme SC]]