View source for 8spk


==Crystal structure of Antarctic PET-degrading enzyme==
<StructureSection load='8spk' size='340' side='right'caption='[[8spk]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8spk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moraxella Moraxella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8SPK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8SPK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8spk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8spk OCA], [https://pdbe.org/8spk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8spk RCSB], [https://www.ebi.ac.uk/pdbsum/8spk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8spk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LIP1_MORS1 LIP1_MORS1] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Several hydrolases have been described to degrade polyethylene terephthalate (PET) at moderate temperatures ranging from 25 degrees C to 40 degrees C. These mesophilic PET hydrolases (PETases) are less efficient in degrading this plastic polymer than their thermophilic homologs and have, therefore, been the subject of many protein engineering campaigns. However, enhancing their enzymatic activity through rational design or directed evolution poses a formidable challenge due to the need for exploring a large number of mutations. Additionally, evaluating the improvements in both activity and stability requires screening numerous variants, either individually or using high-throughput screening methods. Here, we utilize instead the design of chimeras as a protein engineering strategy to increase the activity and stability of Mors1, an Antarctic PETase active at 25 degrees C. First, we obtained the crystal structure of Mors1 at 1.6 A resolution, which we used as a scaffold for structure- and sequence-based chimeric design. Then, we designed a Mors1 chimera via loop exchange of a highly divergent active site loop from the thermophilic leaf-branch compost cutinase (LCC) into the equivalent region in Mors1. After restitution of an active site disulfide bond into this chimera, the enzyme exhibited a shift in optimal temperature for activity to 45 degrees C and an increase in fivefold in PET hydrolysis when compared with wild-type Mors1 at 25 degrees C. Our results serve as a proof of concept of the utility of chimeric design to further improve the activity and stability of PETases active at moderate temperatures.

Engineering the catalytic activity of an Antarctic PET-degrading enzyme by loop exchange.,Blazquez-Sanchez P, Vargas JA, Furtado AA, Grinen A, Leonardo DA, Sculaccio SA, Pereira HD, Sonnendecker C, Zimmermann W, Diez B, Garratt RC, Ramirez-Sarmiento CA Protein Sci. 2023 Sep;32(9):e4757. doi: 10.1002/pro.4757. PMID:37574805<ref>PMID:37574805</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8spk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Moraxella]]
[[Category: Blazquez-Sanchez P]]
[[Category: Diez B]]
[[Category: Furtado AA]]
[[Category: Garratt RC]]
[[Category: Grinen A]]
[[Category: Leonardo DA]]
[[Category: Pereira HM]]
[[Category: Ramirez-Sarmiento CA]]
[[Category: Sculaccio SA]]
[[Category: Vargas JA]]