View source for 8tfi


==tRNA 2'-phosphotransferase (Tpt1) from Pyrococcus horikoshii==
<StructureSection load='8tfi' size='340' side='right'caption='[[8tfi]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8tfi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TFI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tfi OCA], [https://pdbe.org/8tfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tfi RCSB], [https://www.ebi.ac.uk/pdbsum/8tfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tfi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KPTA_PYRHO KPTA_PYRHO] Removes the 2'-phosphate from RNA via an intermediate in which the phosphate is ADP-ribosylated by NAD followed by a presumed transesterification to release the RNA and generate ADP-ribose 1''-2''-cyclic phosphate (APPR>P). May function as an ADP-ribosylase (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Tpt1 is an essential agent of fungal and plant tRNA splicing that removes an internal RNA 2'-phosphate generated by tRNA ligase. Tpt1 also removes the 2'-phosphouridine mark installed by Ark1 kinase in the V-loop of archaeal tRNAs. Tpt1 performs a two-step reaction in which the 2'-PO(4) attacks NAD(+) to form an RNA-2'-phospho-(ADP-ribose) intermediate, and transesterification of the ADP-ribose O2'' to the RNA 2'-phosphodiester yields 2'-OH RNA and ADP-ribose-1'',2''-cyclic phosphate. Here, we present structures of archaeal Tpt1 enzymes, captured as product complexes with ADP-ribose-1''-PO(4), ADP-ribose-2''-PO(4), and 2'-OH RNA, and as substrate complexes with 2',5'-ADP and NAD(+), that illuminate 2'-PO(4) junction recognition and catalysis. We show that archaeal Tpt1 enzymes can use the 2'-PO(4)-containing metabolites NADP(+) and NADPH as substrates for 2'-PO(4) transfer to NAD(+). A role in 2'-phospho-NADP(H) dynamics provides a rationale for the prevalence of Tpt1 in taxa that lack a capacity for internal RNA 2'-phosphorylation.

Structural basis for Tpt1-catalyzed 2'-PO(4) transfer from RNA and NADP(H) to NAD().,Jacewicz A, Dantuluri S, Shuman S Proc Natl Acad Sci U S A. 2023 Oct 31;120(44):e2312999120. doi: , 10.1073/pnas.2312999120. Epub 2023 Oct 26. PMID:37883434<ref>PMID:37883434</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8tfi" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pyrococcus horikoshii OT3]]
[[Category: Dantuluri S]]
[[Category: Jacewicz A]]
[[Category: Shuman S]]