View source for 8tju


==Tetrahymena Ribozyme scaffolded TABV xrRNA==
<StructureSection load='8tju' size='340' side='right'caption='[[8tju]], [[Resolution|resolution]] 3.46&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8tju]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tamana_bat_virus Tamana bat virus] and [https://en.wikipedia.org/wiki/Tetrahymena Tetrahymena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TJU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TJU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.46&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tju OCA], [https://pdbe.org/8tju PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tju RCSB], [https://www.ebi.ac.uk/pdbsum/8tju PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tju ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Single-particle cryo-electron microscopy (cryo-EM) can reveal the structures of large and often dynamic molecules, but smaller biomolecules (&lt;/=50 kDa) remain challenging targets due to their intrinsic low signal to noise ratio. Methods to help resolve small proteins have been applied but development of similar approaches to aid in structural determination of small, structured RNA elements have lagged. Here, we present a scaffold-based approach that we used to recover maps of sub-25 kDa RNA domains to 4.5-5.0 A. While lacking the detail of true high-resolution maps, these maps are suitable for model building and preliminary structure determination. We demonstrate this method helped faithfully recover the structure of several RNA elements of known structure, and that it promises to be generalized to other RNAs without disturbing their native fold. This approach may streamline the sample preparation process and reduce the optimization required for data collection. This first-generation scaffold approach provides a robust system to aid in RNA structure determination by cryo-EM and lays the groundwork for further scaffold optimization to achieve higher resolution.

A generalizable scaffold-based approach for structure determination of RNAs by cryo-EM.,Langeberg CJ, Kieft JS Nucleic Acids Res. 2023 Oct 4:gkad784. doi: 10.1093/nar/gkad784. PMID:37791881<ref>PMID:37791881</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8tju" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribozyme 3D structures|Ribozyme 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Tamana bat virus]]
[[Category: Tetrahymena]]
[[Category: Kieft JS]]
[[Category: Langeberg CJ]]