View source for 8tjv


==Tetrahymena Ribozyme scaffolded Fluoride riboswitch==
<StructureSection load='8tjv' size='340' side='right'caption='[[8tjv]], [[Resolution|resolution]] 2.99&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8tjv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena Tetrahymena] and [https://en.wikipedia.org/wiki/Thermotoga_petrophila Thermotoga petrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TJV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.99&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F:FLUORIDE+ION'>F</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tjv OCA], [https://pdbe.org/8tjv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tjv RCSB], [https://www.ebi.ac.uk/pdbsum/8tjv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tjv ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Single-particle cryo-electron microscopy (cryo-EM) can reveal the structures of large and often dynamic molecules, but smaller biomolecules (&lt;/=50 kDa) remain challenging targets due to their intrinsic low signal to noise ratio. Methods to help resolve small proteins have been applied but development of similar approaches to aid in structural determination of small, structured RNA elements have lagged. Here, we present a scaffold-based approach that we used to recover maps of sub-25 kDa RNA domains to 4.5-5.0 A. While lacking the detail of true high-resolution maps, these maps are suitable for model building and preliminary structure determination. We demonstrate this method helped faithfully recover the structure of several RNA elements of known structure, and that it promises to be generalized to other RNAs without disturbing their native fold. This approach may streamline the sample preparation process and reduce the optimization required for data collection. This first-generation scaffold approach provides a robust system to aid in RNA structure determination by cryo-EM and lays the groundwork for further scaffold optimization to achieve higher resolution.

A generalizable scaffold-based approach for structure determination of RNAs by cryo-EM.,Langeberg CJ, Kieft JS Nucleic Acids Res. 2023 Oct 4:gkad784. doi: 10.1093/nar/gkad784. PMID:37791881<ref>PMID:37791881</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8tjv" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribozyme 3D structures|Ribozyme 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Tetrahymena]]
[[Category: Thermotoga petrophila]]
[[Category: Kieft JS]]
[[Category: Langeberg CJ]]