View source for 8to0


==48-nm repeating structure of doublets from mouse sperm flagella==
<StructureSection load='8to0' size='340' side='right'caption='[[8to0]], [[Resolution|resolution]] 7.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8to0]] is a 348 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TO0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8to0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8to0 OCA], [https://pdbe.org/8to0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8to0 RCSB], [https://www.ebi.ac.uk/pdbsum/8to0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8to0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CFA95_MOUSE CFA95_MOUSE] Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating.[UniProtKB:Q32L77]
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== Publication Abstract from PubMed ==
To understand the molecular mechanisms of cellular pathways, contemporary workflows typically require multiple techniques to identify proteins, track their localization, and determine their structures in vitro. Here, we combined cellular cryoelectron tomography (cryo-ET) and AlphaFold2 modeling to address these questions and understand how mammalian sperm are built in situ. Our cellular cryo-ET and subtomogram averaging provided 6.0-A reconstructions of axonemal microtubule structures. The well-resolved tertiary structures allowed us to unbiasedly match sperm-specific densities with 21,615 AlphaFold2-predicted protein models of the mouse proteome. We identified Tektin 5, CCDC105, and SPACA9 as novel microtubule-associated proteins. These proteins form an extensive interaction network crosslinking the lumen of axonemal doublet microtubules, suggesting their roles in modulating the mechanical properties of the filaments. Indeed, Tekt5 -/- sperm possess more deformed flagella with 180 degrees bends. Together, our studies presented a cellular visual proteomics workflow and shed light on the in vivo functions of Tektin 5.

De novo protein identification in mammalian sperm using in situ cryoelectron tomography and AlphaFold2 docking.,Chen Z, Shiozaki M, Haas KM, Skinner WM, Zhao S, Guo C, Polacco BJ, Yu Z, Krogan NJ, Lishko PV, Kaake RM, Vale RD, Agard DA Cell. 2023 Oct 19:S0092-8674(23)01038-3. doi: 10.1016/j.cell.2023.09.017. PMID:37865089<ref>PMID:37865089</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8to0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Agard DA]]
[[Category: Chen Z]]
[[Category: Guo C]]
[[Category: Hass KM]]
[[Category: Kaake RM]]
[[Category: Krogan NJ]]
[[Category: Polacco BJ]]
[[Category: Shiozak M]]
[[Category: Skinner W]]
[[Category: Vale RD]]
[[Category: Yu Z]]
[[Category: Zhao S]]