View source for 8tum


==Type IV pilus from Pseudomonas PAO1 strain==
<StructureSection load='8tum' size='340' side='right'caption='[[8tum]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8tum]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TUM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tum OCA], [https://pdbe.org/8tum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tum RCSB], [https://www.ebi.ac.uk/pdbsum/8tum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tum ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PILA_PSEAE PILA_PSEAE] Major component of the type IV pilus (T4P) that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, and twitching, a form of surface-associated motility facilitated by cycles of extension, adhesion, and retraction of T4P fibers (PubMed:26041805, PubMed:31431558, PubMed:9282737). In addition, plays a critical role in type II secretion of exoenzymes by interacting with specific components such as XcpT or XcpU (PubMed:9282737). Modulates host calcium signaling through interaction with host calcium-modulating cyclophilin ligand (PubMed:23266901).<ref>PMID:23266901</ref> <ref>PMID:26041805</ref> <ref>PMID:31431558</ref> <ref>PMID:9282737</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The retractile type IV pilus (T4P) is important for virulence of the opportunistic human pathogen Pseudomonas aeruginosa. The single-stranded RNA (ssRNA) phage PP7 binds to T4P and is brought to the cell surface through pilus retraction. Using fluorescence microscopy, we discovered that PP7 detaches T4P, which impairs cell motility and restricts the pathogen's virulence. Using cryo-electron microscopy, mutagenesis, optical trapping, and Langevin dynamics simulation, we resolved the structure of PP7, T4P, and the PP7/T4P complex and showed that T4P detachment is driven by the affinity between the phage maturation protein and its bound pilin, plus the pilus retraction force and speed, and pilus bending. Pilus detachment may be widespread among other ssRNA phages and their retractile pilus systems and offers new prospects for antibacterial prophylaxis and therapeutics.

Removal of Pseudomonas type IV pili by a small RNA virus.,Thongchol J, Yu Z, Harb L, Lin Y, Koch M, Theodore M, Narsaria U, Shaevitz J, Gitai Z, Wu Y, Zhang J, Zeng L Science. 2024 Apr 5;384(6691):eadl0635. doi: 10.1126/science.adl0635. Epub 2024 , Apr 5. PMID:38574145<ref>PMID:38574145</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8tum" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Thongchol J]]
[[Category: Zeng L]]
[[Category: Zhang J]]