View source for 8tw3


==hMPV fusion protein complexed with single domain antibodies sdHMPV16 and sdHMPV12==
<StructureSection load='8tw3' size='340' side='right'caption='[[8tw3]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8tw3]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_metapneumovirus_A Human metapneumovirus A] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TW3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tw3 OCA], [https://pdbe.org/8tw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tw3 RCSB], [https://www.ebi.ac.uk/pdbsum/8tw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tw3 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Human metapneumovirus (hMPV) is an important respiratory pathogen for which no licensed antivirals or vaccines exist. Single-domain antibodies represent promising antiviral biologics that can be easily produced and formatted. We describe the isolation and detailed characterization of two hMPV-neutralizing single-domain antibodies that are directed against the fusion protein F. One of these single-domain antibodies broadly neutralizes hMPV A and B strains, can prevent proteolytic maturation of F, and binds to an epitope in the F trimer interface. This suggests that hMPV pre-F undergoes trimer opening or "breathing" on infectious virions, exposing a vulnerable site for neutralizing antibodies. Finally, we show that this single-domain antibody, fused to a human IgG1 Fc, can protect cotton rats against hMPV replication, an important finding for potential future clinical applications.

A neutralizing single-domain antibody that targets the trimer interface of the human metapneumovirus fusion protein.,Ballegeer M, van Scherpenzeel RC, Delgado T, Iglesias-Caballero M, Garcia Barreno B, Pandey S, Rush SA, Kolkman JA, Mas V, McLellan JS, Saelens X mBio. 2024 Jan 16;15(1):e0212223. doi: 10.1128/mbio.02122-23. Epub 2023 Dec 20. PMID:38117059<ref>PMID:38117059</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8tw3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human metapneumovirus A]]
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: McLellan JS]]
[[Category: Rush SA]]