View source for 8txb


==Characterization of the Chlamydomonas Flagellar Mastigoneme Filament Structure at 3.9A==
<StructureSection load='8txb' size='340' side='right'caption='[[8txb]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8txb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TXB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8txb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8txb OCA], [https://pdbe.org/8txb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8txb RCSB], [https://www.ebi.ac.uk/pdbsum/8txb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8txb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A8J9H7_CHLRE A8J9H7_CHLRE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mastigonemes are thread-like structures adorning the flagella of protists. In Chlamydomonas reinhardtii, filamentous mastigonemes find their roots in the flagella's distal region, associated with the channel protein PKD2, implying their potential contribution to external signal sensing and flagellar motility control. Here, we present the single-particle cryo-electron microscopy structure of the mastigoneme at 3.4 A. The filament unit, MST1, consists of nine immunoglobulin-like domains and six Sushi domains, trailed by an elastic polyproline-II helix. Our structure demonstrates that MST1 subunits are periodically assembled to form a centrosymmetric, non-polar filament. Intriguingly, numerous clustered disulfide bonds within a ladder-like spiral configuration underscore structural resilience. While defects in the mastigoneme structure did not noticeably affect general attributes of cell swimming, they did impact specific swimming properties, particularly under varied environmental conditions such as redox shifts and heightened viscosity. Our findings illuminate the potential role of mastigonemes in flagellar motility and suggest their involvement in diverse environmental responses.

Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes.,Wang Y, Yang J, Hu F, Yang Y, Huang K, Zhang K J Cell Biol. 2023 Dec 4;222(12):e202301066. doi: 10.1083/jcb.202301066. Epub 2023 , Oct 26. PMID:37882754<ref>PMID:37882754</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8txb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Chlamydomonas reinhardtii]]
[[Category: Large Structures]]
[[Category: Kai Z]]
[[Category: Yue W]]