View source for 8uc3


==Cryo-EM structure of the AlbAB cyclodipeptide oxidase enzyme filament==
<StructureSection load='8uc3' size='340' side='right'caption='[[8uc3]], [[Resolution|resolution]] 2.78&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8uc3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_noursei_ATCC_11455 Streptomyces noursei ATCC 11455]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8UC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8UC3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.78&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8uc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8uc3 OCA], [https://pdbe.org/8uc3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8uc3 RCSB], [https://www.ebi.ac.uk/pdbsum/8uc3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8uc3 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Modified cyclic dipeptides represent a widespread class of secondary metabolites with diverse pharmacological activities, including antibacterial, antifungal, and antitumor. Here, we report the structural characterization of the Streptomyces noursei enzyme AlbAB, a cyclodipeptide oxidase (CDO) carrying out alpha,beta-dehydrogenations during the biosynthesis of the antibiotic albonoursin. We show that AlbAB is a megadalton heterooligomeric enzyme filament containing covalently bound flavin mononucleotide cofactors. We highlight that AlbAB filaments consist of alternating dimers of AlbA and AlbB and that enzyme activity is crucially dependent on filament formation. We show that AlbA-AlbB interactions are highly conserved suggesting that other CDO-like enzymes are likely enzyme filaments. As CDOs have been employed in the structural diversification of cyclic dipeptides, our results will be useful for future applications of CDOs in biocatalysis and chemoenzymatic synthesis.

Cyclodipeptide oxidase is an enzyme filament.,Andreas MP, Giessen TW Nat Commun. 2024 Apr 27;15(1):3574. doi: 10.1038/s41467-024-48030-9. PMID:38678027<ref>PMID:38678027</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8uc3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces noursei ATCC 11455]]
[[Category: Andreas MP]]
[[Category: Giessen TW]]