View source for 8ukc


==Solution NMR Structure of the lasso peptide chlorolassin==
<StructureSection load='8ukc' size='340' side='right'caption='[[8ukc]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ukc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_jiangxiensis Lentzea jiangxiensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8UKC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8UKC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CW:5-CHLORO-L-TRYPTOPHAN'>5CW</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ukc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ukc OCA], [https://pdbe.org/8ukc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ukc RCSB], [https://www.ebi.ac.uk/pdbsum/8ukc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ukc ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Lasso peptides are a class of ribosomally synthesized and post-translationally modified peptides (RiPPs) defined by a macrolactam linkage between the N-terminus and the side chain of an internal aspartic acid or glutamic acid residue. Instead of adopting a branched-cyclic conformation, lasso peptides are "threaded", with the C-terminal tail passing through the macrocycle to present a kinetically trapped rotaxane conformation. The availability of enhanced bioinformatics methods has led to a significant increase in the number of secondary modifications found on lasso peptides. To uncover new ancillary modifications in a targeted manner, a bioinformatic strategy was developed to discover lasso peptides with modifications to tryptophan. This effort identified numerous putative lasso peptide biosynthetic gene clusters with core regions of the precursor peptides enriched in tryptophan. Parsing of these tryptophan (Trp)-rich biosynthetic gene clusters uncovered several putative ancillary modifying enzymes, including halogenases and dimethylallyltransferases expected to act upon Trp. Characterization of two gene products yielded a lasso peptide with two 5-Cl-Trp modifications (chlorolassin) and another bearing 5-dimethylallyl-Trp and 2,3-didehydro-Tyr modifications (wygwalassin). Bioinformatic analysis of the requisite halogenase and dimethylallyltransferase revealed numerous other putative Trp-modified lasso peptides that remain uncharacterized. We anticipate that the Trp-centric strategy reported herein may be useful in discovering ancillary modifications for other RiPP classes and, more generally, guide the functional prediction of enzymes that act on specific amino acids.

Tryptophan-Centric Bioinformatics Identifies New Lasso Peptide Modifications.,Harris LA, Saad H, Shelton KE, Zhu L, Guo X, Mitchell DA Biochemistry. 2024 Apr 2;63(7):865-879. doi: 10.1021/acs.biochem.4c00035. Epub , 2024 Mar 18. PMID:38498885<ref>PMID:38498885</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 8ukc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lentzea jiangxiensis]]
[[Category: Harris LA]]
[[Category: Mitchell DA]]
[[Category: Saad H]]
[[Category: Shelton KE]]
[[Category: Zhu L]]