View source for 8ukg


==Solution NMR structure of the lasso peptide wygwalassin-A1==
<StructureSection load='8ukg' size='340' side='right'caption='[[8ukg]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ukg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_katrae Streptomyces katrae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8UKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8UKG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PQ4:(2Z)-2-amino-3-(4-hydroxyphenyl)prop-2-enoic+acid'>PQ4</scene>, <scene name='pdbligand=WWB:5-(3-methylbut-2-en-1-yl)-L-tryptophan'>WWB</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ukg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ukg OCA], [https://pdbe.org/8ukg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ukg RCSB], [https://www.ebi.ac.uk/pdbsum/8ukg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ukg ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Lasso peptides are a class of ribosomally synthesized and post-translationally modified peptides (RiPPs) defined by a macrolactam linkage between the N-terminus and the side chain of an internal aspartic acid or glutamic acid residue. Instead of adopting a branched-cyclic conformation, lasso peptides are "threaded", with the C-terminal tail passing through the macrocycle to present a kinetically trapped rotaxane conformation. The availability of enhanced bioinformatics methods has led to a significant increase in the number of secondary modifications found on lasso peptides. To uncover new ancillary modifications in a targeted manner, a bioinformatic strategy was developed to discover lasso peptides with modifications to tryptophan. This effort identified numerous putative lasso peptide biosynthetic gene clusters with core regions of the precursor peptides enriched in tryptophan. Parsing of these tryptophan (Trp)-rich biosynthetic gene clusters uncovered several putative ancillary modifying enzymes, including halogenases and dimethylallyltransferases expected to act upon Trp. Characterization of two gene products yielded a lasso peptide with two 5-Cl-Trp modifications (chlorolassin) and another bearing 5-dimethylallyl-Trp and 2,3-didehydro-Tyr modifications (wygwalassin). Bioinformatic analysis of the requisite halogenase and dimethylallyltransferase revealed numerous other putative Trp-modified lasso peptides that remain uncharacterized. We anticipate that the Trp-centric strategy reported herein may be useful in discovering ancillary modifications for other RiPP classes and, more generally, guide the functional prediction of enzymes that act on specific amino acids.

Tryptophan-Centric Bioinformatics Identifies New Lasso Peptide Modifications.,Harris LA, Saad H, Shelton KE, Zhu L, Guo X, Mitchell DA Biochemistry. 2024 Apr 2;63(7):865-879. doi: 10.1021/acs.biochem.4c00035. Epub , 2024 Mar 18. PMID:38498885<ref>PMID:38498885</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ukg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces katrae]]
[[Category: Harris LA]]
[[Category: Mitchell DA]]
[[Category: Saad H]]
[[Category: Shelton KE]]
[[Category: Zhu L]]