View source for 8umc


==Deinococcus aerius TR0125 C-glucosyl deglycosidase (CGD), cryo-EM==
<StructureSection load='8umc' size='340' side='right'caption='[[8umc]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8umc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_aerius Deinococcus aerius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8UMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8UMC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8umc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8umc OCA], [https://pdbe.org/8umc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8umc RCSB], [https://www.ebi.ac.uk/pdbsum/8umc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8umc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2I9DAN1_9DEIO A0A2I9DAN1_9DEIO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Plant C-glycosylated aromatic polyketides are important for plant and animal health. These are specialized metabolites that perform functions both within the plant, and in interaction with soil or intestinal microbes. Despite the importance of these plant compounds, there is still limited knowledge of how they are metabolized. The Gram-positive aerobic soil bacterium Deinococcus aerius strain TR0125 and other Deinococcus species thrive in a wide range of harsh environments. In this work, we identified a C-glycoside deglycosylation gene cluster in the genome of D. aerius. The cluster includes three genes coding for a GMC-type oxidoreductase (DaCGO1) that oxidizes the glucosyl C3 position in aromatic C-glucosyl compounds, which in turn provides the substrate for the C-glycoside deglycosidase (DaCGD; composed of alpha+beta subunits) that cleaves the glucosyl-aglycone C-C bond. Our results from size-exclusion chromatography, single particle cryo-electron microscopy and X-ray crystallography show that DaCGD is an alpha(2)beta(2) heterotetramer, which represents a novel oligomeric state among bacterial CGDs. Importantly, the high-resolution X-ray structure of DaCGD provides valuable insights into the activation of the catalytic hydroxide ion by Lys261. DaCGO1 is specific for the 6-C-glucosyl flavones isovitexin, isoorientin and the 2-C-glucosyl xanthonoid mangiferin, and the subsequent C-C-bond cleavage by DaCGD generated apigenin, luteolin and norathyriol, respectively. Of the substrates tested, isovitexin was the preferred substrate (DaCGO1, K(m) 0.047 mM, k(cat) 51 min(-1); DaCGO1/DaCGD, K(m) 0.083 mM, k(cat) 0.42 min(-1)).

Structural and Functional Characterization of a Gene Cluster Responsible for Deglycosylation of C-glucosyl Flavonoids and Xanthonoids by Deinococcus aerius.,Furlanetto V, Kalyani DC, Kostelac A, Puc J, Haltrich D, Hallberg BM, Divne C J Mol Biol. 2024 May 1;436(9):168547. doi: 10.1016/j.jmb.2024.168547. Epub 2024 , Mar 18. PMID:38508304<ref>PMID:38508304</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8umc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Deinococcus aerius]]
[[Category: Large Structures]]
[[Category: Divne C]]
[[Category: Furlanetto V]]
[[Category: Hallberg BM]]
[[Category: Haltrich D]]
[[Category: Kalyani DC]]
[[Category: Kostelac A]]