View source for 8uzt


==Mitochondrial single-stranded binding protein bound to DNA==
<StructureSection load='8uzt' size='340' side='right'caption='[[8uzt]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8uzt]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8UZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8UZT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8uzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8uzt OCA], [https://pdbe.org/8uzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8uzt RCSB], [https://www.ebi.ac.uk/pdbsum/8uzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8uzt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SSBP_HUMAN SSBP_HUMAN] This protein binds preferentially and cooperatively to ss-DNA. Probably involved in mitochondrial DNA replication. Associates with mitochondrial DNA.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The mitochondrial single-stranded DNA (ssDNA) binding protein, mtSSB or SSBP1, binds to ssDNA to prevent secondary structures of DNA that could impede downstream replication or repair processes. Clinical mutations in the SSBP1 gene have been linked to a range of mitochondrial disorders affecting nearly all organs and systems. Yet, the molecular determinants governing the interaction between mtSSB and ssDNA have remained elusive. Similarly, the structural interaction between mtSSB and other replisome components, such as the mitochondrial DNA polymerase, Polgamma, has been minimally explored. Here, we determined a 1.9-A X-ray crystallography structure of the human mtSSB bound to ssDNA. This structure uncovered two distinct DNA binding sites, a low-affinity site and a high-affinity site, confirmed through site-directed mutagenesis. The high-affinity binding site encompasses a clinically relevant residue, R38, and a highly conserved DNA base stacking residue, W84. Employing cryo-electron microscopy, we confirmed the tetrameric assembly in solution and capture its interaction with Polgamma. Finally, we derived a model depicting modes of ssDNA wrapping around mtSSB and a region within Polgamma that mtSSB binds.

Structures of the mitochondrial single-stranded DNA binding protein with DNA and DNA polymerase gamma.,Riccio AA, Bouvette J, Pedersen LC, Somai S, Dutcher RC, Borgnia MJ, Copeland WC Nucleic Acids Res. 2024 Aug 6:gkae670. doi: 10.1093/nar/gkae670. PMID:39106165<ref>PMID:39106165</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8uzt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Borgnia JM]]
[[Category: Bouvette J]]
[[Category: Copeland WC]]
[[Category: Pedersen LC]]
[[Category: Riccio AA]]