View source for 8v40


==CryoEM Structure of Diffocin - postcontracted - Collar - final state==
<StructureSection load='8v40' size='340' side='right'caption='[[8v40]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8v40]] is a 42 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile Clostridioides difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8V40 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8V40 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8v40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8v40 OCA], [https://pdbe.org/8v40 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8v40 RCSB], [https://www.ebi.ac.uk/pdbsum/8v40 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8v40 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A031WFC4_CLODI A0A031WFC4_CLODI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Due to envelope differences between Gram-positive and Gram-negative bacteria, engineering precision bactericidal contractile nanomachines requires atomic-level understanding of their structures; however, only those killing Gram-negative bacteria are currently known. Here, we report the atomic structures of an engineered diffocin, a contractile syringe-like molecular machine that kills the Gram-positive bacterium Clostridioides difficile. Captured in one pre-contraction and two post-contraction states, each structure fashions six proteins in the bacteria-targeting baseplate, two proteins in the energy-storing trunk, and a collar linking the sheath with the membrane-penetrating tube. Compared to contractile machines targeting Gram-negative bacteria, major differences reside in the baseplate and contraction magnitude, consistent with target envelope differences. The multifunctional hub-hydrolase protein connects the tube and baseplate and is positioned to degrade peptidoglycan during penetration. The full-length tape measure protein forms a coiled-coil helix bundle homotrimer spanning the entire diffocin. Our study offers mechanical insights and principles for designing potent protein-based precision antibiotics.

Atomic structures of a bacteriocin targeting Gram-positive bacteria.,Cai X, He Y, Yu I, Imani A, Scholl D, Miller JF, Zhou ZH Nat Commun. 2024 Aug 16;15(1):7057. doi: 10.1038/s41467-024-51038-w. PMID:39152109<ref>PMID:39152109</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8v40" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Clostridioides difficile]]
[[Category: Large Structures]]
[[Category: Cai XY]]
[[Category: He Y]]
[[Category: Zhou ZH]]