View source for 8vf5


==CryoEM structure of Ku homodimer in complex with linear DNA==
<StructureSection load='8vf5' size='340' side='right'caption='[[8vf5]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8vf5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv] and [https://en.wikipedia.org/wiki/Saltans_group Saltans group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8VF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8VF5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8vf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8vf5 OCA], [https://pdbe.org/8vf5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8vf5 RCSB], [https://www.ebi.ac.uk/pdbsum/8vf5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8vf5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KU_MYCTU KU_MYCTU] With LigD forms a non-homologous end joining (NHEJ) repair enzyme. Binds linear dsDNA with 5'- and 3'-overhangs but not closed circular dsDNA nor ssDNA. One dimer binds for every 30 bp. Recruits and stimulates the ligase activity of LigD but not of T4 ligase or a human ligase complex (LIG4/XRCC4). Attenuates the 3'- to 5'-exonuclease activity of LigD. Stimulates the template-directed addition of dNTPs by LigD on 5'-overhangs and nuclease activity on 3'-overhangs.<ref>PMID:12215643</ref> <ref>PMID:15499016</ref> <ref>PMID:15778718</ref> <ref>PMID:16023671</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis H37Rv]]
[[Category: Saltans group]]
[[Category: Baral J]]
[[Category: Das AK]]
[[Category: Rouiller I]]