View source for 8vom


==Double alanine Apex domain mutant of bacteriophage P2 central spike protein, membrane-piercing module==
<StructureSection load='8vom' size='340' side='right'caption='[[8vom]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8vom]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_P2 Bacteriophage P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8VOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8VOM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTY:PHOSPHATIDYLETHANOLAMINE'>PTY</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8vom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8vom OCA], [https://pdbe.org/8vom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8vom RCSB], [https://www.ebi.ac.uk/pdbsum/8vom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8vom ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPIKE_BPP2 SPIKE_BPP2] Forms the small spikes on the baseplate that plug the end of the tube before DNA ejection and form a channel perforating the host membrane during ejection. Involved in baseplate assembly.<ref>PMID:21821878</ref> <ref>PMID:22325780</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The contractile tail of bacteriophage P2 functions to drive the tail tube across the outer membrane of its host bacterium, a prerequisite event for subsequent translocation of phage genomic DNA into the host cell. The tube is equipped with a spike-shaped protein (product of P2 gene V , gpV or Spike) that contains a membrane-attacking Apex domain carrying a centrally positioned Fe ion. The ion is enclosed in a histidine cage that is formed by three symmetry-related copies of a conserved HxH (histidine, any residue, histidine) sequence motif. Here, we used solution biophysics and X-ray crystallography to characterize the structure and properties of Spike mutants in which the Apex domain was either deleted or its histidine cage was either destroyed or replaced with a hydrophobic core. We found that the Apex domain is not required for the folding of full-length gpV or its middle intertwined beta-helical domain. Furthermore, despite its high conservation, the Apex domain is dispensable for infection in laboratory conditions. Collectively, our results show that the diameter of the Spike but not the nature of its Apex domain determines the efficiency of infection, which further strengthens the earlier hypothesis of a drill bit-like function of the Spike in host envelope disruption.

Function of the bacteriophage P2 baseplate central spike Apex domain in the infection process.,Miller JM, Knyazhanskaya ES, Buth SA, Prokhorov NS, Leiman PG bioRxiv [Preprint]. 2023 Feb 25:2023.02.25.529910. doi: , 10.1101/2023.02.25.529910. PMID:36865152<ref>PMID:36865152</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8vom" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacteriophage P2]]
[[Category: Large Structures]]
[[Category: Leiman PG]]
[[Category: Miller JM]]