View source for 8vsh


==Crystal structure of Shewanella benthica Group 1 truncated hemoglobin C51S C71S variant with trans heme D==
<StructureSection load='8vsh' size='340' side='right'caption='[[8vsh]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8vsh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_benthica_KT99 Shewanella benthica KT99]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8VSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8VSH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1ADT:{3-[(2R,5R)-9,14-diethenyl-5-hydroxy-5,10,15,19-tetramethyl-5-oxo-4,5-dihydro-3H-spiro[furan-2,4-[21,22,23,24]tetraazapentacyclo[16.2.1.13,6.18,11.113,16]tetracosa[1,3(24),6,8,10,12,14,16(22),17,19]decaen]-20-yl-kappa~4~N~21~,N~22~,N~23~,N~24~]propanoato}iron'>A1ADT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8vsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8vsh OCA], [https://pdbe.org/8vsh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8vsh RCSB], [https://www.ebi.ac.uk/pdbsum/8vsh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8vsh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A9DF82_9GAMM A9DF82_9GAMM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In our continued investigations of microbial globins, we solved the structure of a truncated hemoglobin from Shewanella benthica, an obligate psychropiezophilic bacterium. The distal side of the heme active site is lined mostly with hydrophobic residues, with the exception of a tyrosine, Tyr34 (CD1) and a histidine, His24 (B13). We found that purified SbHbN, when crystallized in the ferric form with polyethylene glycol as precipitant, turned into a green color over weeks. The electron density obtained from the green crystals accommodated a trans heme d, a chlorin-type derivative featuring a gamma-spirolactone and a vicinal hydroxyl group on a pyrroline ring. In solution, exposure of the protein to one equivalent of hydrogen peroxide resulted in a similar green color change, but caused by the formation of multiple products. These were oxidation species released on protein denaturation, likely including heme d, and a species with heme covalently attached to the polypeptide. The Tyr34Phe replacement prevented the formation of both heme d and the covalent linkage. The ready modification of heme b by SbHbN expands the range of chemistries supported by the globin fold and offers a route to a novel heme cofactor.

Heme d formation in a Shewanella benthica hemoglobin.,Martinez Grundman JE, Schultz TD, Schlessman JL, Liu K, Johnson EA, Lecomte JTJ J Inorg Biochem. 2024 Jun 27;259:112654. doi: 10.1016/j.jinorgbio.2024.112654. PMID:38959524<ref>PMID:38959524</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8vsh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Shewanella benthica KT99]]
[[Category: Lecomte JTJ]]
[[Category: Martinez JE]]
[[Category: Schlessman JL]]
[[Category: Schultz TD]]
[[Category: Siegler MA]]