View source for 8wcl


==FCP pentamer in Chaetoceros gracilis==
<StructureSection load='8wcl' size='340' side='right'caption='[[8wcl]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8wcl]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetoceros_neogracilis Chaetoceros neogracilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WCL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A86:(3S,3S,5R,5R,6S,6R,8R)-3,5-dihydroxy-8-oxo-6,7-didehydro-5,5,6,6,7,8-hexahydro-5,6-epoxy-beta,beta-caroten-3-+yl+acetate'>A86</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=KC1:Chlorophyll+c1'>KC1</scene>, <scene name='pdbligand=KC2:Chlorophyll+c2'>KC2</scene>, <scene name='pdbligand=LMG:1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE'>LMG</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wcl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wcl OCA], [https://pdbe.org/8wcl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wcl RCSB], [https://www.ebi.ac.uk/pdbsum/8wcl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wcl ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Diatoms, a group of prevalent marine algae, significantly contribute to global primary productivity. Their substantial biomass is linked to enhanced absorption of blue-green light underwater, facilitated by fucoxanthin chlorophyll a/c-binding proteins (FCPs), exhibiting oligomeric diversity across diatom species. Utilizing mild CN-PAGE analysis on solubilized thylakoid membranes, we displayed monomeric, dimeric, trimeric, tetrameric and pentameric FCPs in diatoms. Mass spectrometry analysis revealed each oligomeric FCP has specific protein compositions, constituting a large Lhcf family of FCP antennas. In addition, we resolved the structures of Thalassiosira pseudonana FCP (Tp-FCP) homotrimer and Chaetoceros gracilis FCP (Cg-FCP) pentamer by cryo-electron microscopy at 2.73 A and 2.65 A resolutions, respectively. The distinct pigment composition and organization in various oligomeric FCPs change their blue-green light-harvesting, excitation energy transfer pathways. In comparison to dimeric and trimeric FCPs, Cg-FCP tetramer and Cg-FCP pentamer exhibit stronger absorption by Chls c, red-shifted and broader Chl a fluorescence emission, as well as more robust circular dichroism signals originating from Chl a-carotenoid dimers. These spectroscopic characteristics indicate that Chl a molecules in Cg-FCP tetramer and Cg-FCP pentamer are more heterogeneous than in both dimers and Tp-FCP trimer. The structural and spectroscopic insights provided by this study contribute to a better understanding of the mechanisms that empower diatoms to adapt to fluctuating light environments.

Structural and spectroscopic insights into fucoxanthin chlorophyll a/c-binding proteins of diatoms in diverse oligomeric states.,Zhou C, Feng Y, Li Z, Shen L, Li X, Wang Y, Han G, Kuang T, Liu C, Shen JR, Wang W Plant Commun. 2024 Jul 17:101041. doi: 10.1016/j.xplc.2024.101041. PMID:39030906<ref>PMID:39030906</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8wcl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Chaetoceros neogracilis]]
[[Category: Large Structures]]
[[Category: Feng Y]]
[[Category: Li Z]]
[[Category: Liu C]]
[[Category: Shen J-R]]
[[Category: Wang W]]
[[Category: Zhou C]]