View source for 8wkp


==Structural basis of translation inhibition by a valine tRNA-derived fragment==
<StructureSection load='8wkp' size='340' side='right'caption='[[8wkp]], [[Resolution|resolution]] 4.62&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8wkp]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius_DSM_639 Sulfolobus acidocaldarius DSM 639]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WKP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.62&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wkp OCA], [https://pdbe.org/8wkp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wkp RCSB], [https://www.ebi.ac.uk/pdbsum/8wkp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wkp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS4E_SULAC RS4E_SULAC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Translational regulation by non-coding RNAs is a mechanism commonly used by cells to fine-tune gene expression. A fragment derived from an archaeal valine tRNA (Val-tRF) has been previously identified to bind the small subunit of the ribosome and inhibit translation in Haloferax volcanii Here, we present three cryo-electron microscopy structures of Val-tRF bound to the small subunit of Sulfolobus acidocaldarius ribosomes at resolutions between 4.02 and 4.53 A. Within these complexes, Val-tRF was observed to bind to conserved RNA-interacting sites, including the ribosomal decoding center. The binding of Val-tRF destabilizes helices h24, h44, and h45 and the anti-Shine-Dalgarno sequence of 16S rRNA. The binding position of this molecule partially overlaps with the translation initiation factor aIF1A and occludes the mRNA P-site codon. Moreover, we found that the binding of Val-tRF is associated with steric hindrance of the H69 base of 23S rRNA in the large ribosome subunit, thereby preventing 70S assembly. Our data exemplify how tRNA-derived fragments bind to ribosomes and provide new insights into the mechanisms underlying translation inhibition by Val-tRFs.

Structural basis of translation inhibition by a valine tRNA-derived fragment.,Wu Y, Ni MT, Wang YH, Wang C, Hou H, Zhang X, Zhou J Life Sci Alliance. 2024 Apr 10;7(6):e202302488. doi: 10.26508/lsa.202302488. , Print 2024 Jun. PMID:38599770<ref>PMID:38599770</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8wkp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Sulfolobus acidocaldarius DSM 639]]
[[Category: Wang YH]]
[[Category: Zhou J]]