View source for 8wks


==Cryo-EM structure of DSR2-TUBE complex==
<StructureSection load='8wks' size='340' side='right'caption='[[8wks]], [[Resolution|resolution]] 3.58&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8wks]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._natto_BEST195 Bacillus subtilis subsp. natto BEST195] and [https://en.wikipedia.org/wiki/Siphoviridae_sp._ct0106 Siphoviridae sp. ct0106]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WKS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.58&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wks OCA], [https://pdbe.org/8wks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wks RCSB], [https://www.ebi.ac.uk/pdbsum/8wks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wks ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D4G637_BACNB D4G637_BACNB] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Defense-associated sirtuin 2 (DSR2) systems are widely distributed across prokaryotic genomes, providing robust protection against phage infection. DSR2 recognizes phage tail tube proteins and induces abortive infection by depleting intracellular NAD(+), a process that is counteracted by another phage-encoded protein, DSR Anti Defense 1 (DSAD1). Here, we present cryo-EM structures of Bacillus subtilis DSR2 in its apo, Tube-bound, and DSAD1-bound states. DSR2 assembles into an elongated tetramer, with four NADase catalytic modules clustered in the center and the regulatory-sensing modules distributed at four distal corners. Interestingly, monomeric Tube protein, rather than its oligomeric states, docks at each corner of the DSR2 tetramer to form a 4:4 DSR2-Tube assembly, which is essential for DSR2 NADase activity. DSAD1 competes with Tube for binding to DSR2 by occupying an overlapping region, thereby inhibiting DSR2 immunity. Thus, our results provide important insights into the assembly, activation and inhibition of the DSR2 anti-phage defense system.

Molecular basis of bacterial DSR2 anti-phage defense and viral immune evasion.,Huang J, Zhu K, Gao Y, Ye F, Li Z, Ge Y, Liu S, Yang J, Gao A Nat Commun. 2024 May 10;15(1):3954. doi: 10.1038/s41467-024-48291-4. PMID:38729958<ref>PMID:38729958</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8wks" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Bacillus subtilis subsp. natto BEST195]]
[[Category: Large Structures]]
[[Category: Siphoviridae sp. ct0106]]
[[Category: Gao A]]
[[Category: Huang J]]
[[Category: Zhu K]]