View source for 8wq4


==Structural basis of translation inhibition by a valine tRNA-derived fragment==
<StructureSection load='8wq4' size='340' side='right'caption='[[8wq4]], [[Resolution|resolution]] 4.53&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8wq4]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius_DSM_639 Sulfolobus acidocaldarius DSM 639]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WQ4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.53&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wq4 OCA], [https://pdbe.org/8wq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wq4 RCSB], [https://www.ebi.ac.uk/pdbsum/8wq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wq4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS4_SULAC RS4_SULAC] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.  With S5 and S12 plays an important role in translational accuracy.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Translational regulation by non-coding RNAs is a mechanism commonly used by cells to fine-tune gene expression. A fragment derived from an archaeal valine tRNA (Val-tRF) has been previously identified to bind the small subunit of the ribosome and inhibit translation in Haloferax volcanii Here, we present three cryo-electron microscopy structures of Val-tRF bound to the small subunit of Sulfolobus acidocaldarius ribosomes at resolutions between 4.02 and 4.53 A. Within these complexes, Val-tRF was observed to bind to conserved RNA-interacting sites, including the ribosomal decoding center. The binding of Val-tRF destabilizes helices h24, h44, and h45 and the anti-Shine-Dalgarno sequence of 16S rRNA. The binding position of this molecule partially overlaps with the translation initiation factor aIF1A and occludes the mRNA P-site codon. Moreover, we found that the binding of Val-tRF is associated with steric hindrance of the H69 base of 23S rRNA in the large ribosome subunit, thereby preventing 70S assembly. Our data exemplify how tRNA-derived fragments bind to ribosomes and provide new insights into the mechanisms underlying translation inhibition by Val-tRFs.

Structural basis of translation inhibition by a valine tRNA-derived fragment.,Wu Y, Ni MT, Wang YH, Wang C, Hou H, Zhang X, Zhou J Life Sci Alliance. 2024 Apr 10;7(6):e202302488. doi: 10.26508/lsa.202302488. , Print 2024 Jun. PMID:38599770<ref>PMID:38599770</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8wq4" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Sulfolobus acidocaldarius DSM 639]]
[[Category: Wang YH]]
[[Category: Zhou J]]