View source for 8wu6


==Structure of a Nerylneryl Diphosphate Synthase from Solanum lycopersicum==
<StructureSection load='8wu6' size='340' side='right'caption='[[8wu6]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8wu6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_lycopersicum Solanum lycopersicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WU6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WU6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wu6 OCA], [https://pdbe.org/8wu6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wu6 RCSB], [https://www.ebi.ac.uk/pdbsum/8wu6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wu6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CPT2_SOLLC CPT2_SOLLC] Uses dimethylallyl diphosphate and isopentenyl diphosphate to catalyze the cis-prenyl chain elongation and produce the 20 carbon product nerylneryl diphosphate.<ref>PMID:23134568</ref> <ref>PMID:23757397</ref> <ref>PMID:25786135</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
cis-Prenyltransferases (cis-PTs) catalyze the sequential head-to-tail condensation of isopentenyl diphosphate (IPP) to allylic diphosphates, producing mixed E-Z prenyl diphosphates of varying lengths; yet, the specific enzymes synthesizing cis-C25 prenyl diphosphates have not been identified. In this study, we present the discovery and characterization of a cis-geranylfarnesyl diphosphate synthase (ScGFPPS) from Streptomyces clavuligerus. This enzyme demonstrates high catalytic proficiency in generating six distinct cis-polyisoprenoids, including three C25 and three C20 variants. We further determine the crystal structure of ScGFPPS. Additionally, we unveil the crystal structure of nerylneryl diphosphate synthase (NNPS), known for synthesizing an all-cis-C20 polyisoprenoid. Comparative structural analysis of ScGFPPS and NNPS has identified key differences that influence product specificity. Through site-directed mutagenesis, we have identified eight single mutations that significantly refine ScGFPPS's selectivity for cis-polyisoprenoids. Our findings not only expand the functional spectrum of cis-PTs but also provide a structural comparison strategy in cis-PTs engineering.

Discovery, Structure, and Engineering of a cis-Geranylfarnesyl Diphosphate Synthase.,Li FR, Wang Q, Pan X, Xu HM, Dong LB Angew Chem Int Ed Engl. 2024 Apr 23:e202401669. doi: 10.1002/anie.202401669. PMID:38651244<ref>PMID:38651244</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8wu6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Solanum lycopersicum]]
[[Category: Dong LB]]
[[Category: Li FR]]
[[Category: Pan XM]]
[[Category: Wang QL]]