View source for 8wzl


==Arabidopsis thaliana Histone deacetylase 7 (HDA7)==
<StructureSection load='8wzl' size='340' side='right'caption='[[8wzl]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8wzl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WZL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WZL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wzl OCA], [https://pdbe.org/8wzl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wzl RCSB], [https://www.ebi.ac.uk/pdbsum/8wzl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wzl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HDA7_ARATH HDA7_ARATH] Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. May be involved in flowering induction. Histone deacetylases act via the formation of large multiprotein complexes.<ref>PMID:16699543</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Histone deacetylases (HDACs), responsible for the removal of acetyl groups from histone tails, are important epigenetic factors. They play a critical role in the regulation of gene expression and are significant in the context of plant growth and development. The Rpd3/Hda1 family of HDACs is reported to regulate key biological processes in plants, such as stress response, seed, embryonic, and floral development. Here, we characterized Arabidopsis thaliana HDA7, a Class I, Rpd3/Hda1 family HDAC. SAXS and AUC results show that the recombinantly expressed and purified histone deacetylase domain of AtHDA7 exists as a monomer in solution. Further, the crystal structure showed AtHDA7 to fold into the typical alpha/beta arginase fold, characteristic of Rpd3/Hda1 family HDACs. Sequence analysis revealed that the Asp and His residues of the catalytic 'XDXH' motif present in functional Rpd3/Hda1 family HDACs are mutated to Gly and Pro, respectively, in AtHDA7, suggesting that it might be catalytically inactive. The Asp and His residues are important for Zn(2+)-binding. Not surprisingly, the crystal structure did not have Zn(2+) bound in the catalytic pocket, which is essential for the HDAC activity. Further, our in vitro activity assay revealed AtHDA7 to be inactive as an HDAC. A search in the sequence databases suggested that homologs of AtHDA7 are found exclusively in the Brassicaceae family to which Arabidopsis belongs. It is possible that HDA7 descended from HDA6 through whole genome duplication and triplication events during evolution, as suggested in a previous phylogenetic study.

Structure-function analyses reveal Arabidopsis thaliana HDA7 to be an inactive histone deacetylase.,Saharan K, Baral S, Shaikh NH, Vasudevan D Curr Res Struct Biol. 2024 Feb 23;7:100136. doi: 10.1016/j.crstbi.2024.100136. , eCollection 2024. PMID:38463934<ref>PMID:38463934</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8wzl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Saharan K]]
[[Category: Vasudevan D]]