View source for 8x3g


==Crystal structure of metformin hydrolase from Aminobacter==
<StructureSection load='8x3g' size='340' side='right'caption='[[8x3g]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8x3g]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aminobacter_sp._NyZ550 Aminobacter sp. NyZ550]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8X3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8X3G FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.84&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8x3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8x3g OCA], [https://pdbe.org/8x3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8x3g RCSB], [https://www.ebi.ac.uk/pdbsum/8x3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8x3g ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The biguanide drug metformin is a first-line blood glucose-lowering medication for type 2 diabetes, leading to its presence in the global environment. However, little is known about the fate of metformin by microbial catabolism. Here, we characterize a Ni(2+)-dependent heterohexameric enzyme (MetCaCb) from the ureohydrolase superfamily, catalyzing the hydrolysis of metformin into guanylurea and dimethylamine. Either subunit alone is catalytically inactive, but together they work as an active enzyme highly specific for metformin. The crystal structure of the MetCaCb complex shows the coordination of the binuclear metal cluster only in MetCa, with MetCb as a protein binder of its active cognate. An in-silico search and functional assay discover a group of MetCaCb-like protein pairs exhibiting metformin hydrolase activity in the environment. Our findings not only establish the genetic and biochemical foundation for metformin catabolism but also provide additional insights into the adaption of the ancient enzymes toward newly occurred substrate.

Discovery of a Ni(2+)-dependent heterohexameric metformin hydrolase.,Li T, Xu ZJ, Zhang ST, Xu J, Pan P, Zhou NY Nat Commun. 2024 Jul 20;15(1):6121. doi: 10.1038/s41467-024-50409-7. PMID:39033196<ref>PMID:39033196</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Aminobacter sp. NyZ550]]
[[Category: Large Structures]]
[[Category: Li T]]
[[Category: Zhou NY]]