View source for 8x74


==Crystal structure of ZmHSL1A complexed with mesotrione==
<StructureSection load='8x74' size='340' side='right'caption='[[8x74]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8x74]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8X74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8X74 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.791&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=92L:2-[(4-methylsulfonyl-2-nitro-phenyl)-oxidanyl-methylidene]cyclohexane-1,3-dione'>92L</scene>, <scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8x74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8x74 OCA], [https://pdbe.org/8x74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8x74 RCSB], [https://www.ebi.ac.uk/pdbsum/8x74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8x74 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B6U9L0_MAIZE B6U9L0_MAIZE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Discovering and engineering herbicide-resistant genes is a crucial challenge in crop breeding. This study focuses on the 4-hydroxyphenylpyruvate dioxygenase Inhibitor Sensitive 1-Like (HSL) protein, prevalent in higher plants and exhibiting weak catalytic activity against many beta-triketone herbicides (beta-THs). The crystal structures of maize HSL1A complexed with beta-THs were elucidated, identifying four essential herbicide-binding residues and explaining the weak activity of HSL1A against the herbicides. Utilizing an artificial evolution approach, we developed a series of rice HSL1 mutants targeting the four residues. Then, these mutants were systematically evaluated, identifying the M10 variant as the most effective in modifying beta-THs. The initial active conformation of substrate binding in HSL1 was also revealed from these mutants. Furthermore, overexpression of M10 in rice significantly enhanced resistance to beta-THs, resulting in a notable 32-fold increase in resistance to methyl-benquitrione. In conclusion, the artificially evolved M10 gene shows great potential for the development of herbicide-resistant crops.

An artificially evolved gene for herbicide-resistant rice breeding.,Dong J, Yu XH, Dong J, Wang GH, Wang XL, Wang DW, Yan YC, Xiao H, Ye BQ, Lin HY, Yang GF Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2407285121. doi: , 10.1073/pnas.2407285121. Epub 2024 Aug 12. PMID:39133859<ref>PMID:39133859</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8x74" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Zea mays]]
[[Category: Dong J]]
[[Category: Lin H-Y]]
[[Category: Yang G-F]]