View source for 8xac


==Crystal structure of amidase from Pseudonocardia acaciae==
<StructureSection load='8xac' size='340' side='right'caption='[[8xac]], [[Resolution|resolution]] 3.02&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8xac]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudonocardia_acaciae Pseudonocardia acaciae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XAC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.02&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xac OCA], [https://pdbe.org/8xac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xac RCSB], [https://www.ebi.ac.uk/pdbsum/8xac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xac ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Hydrazidase from Microbacterium hydrocarbonoxydans was revealed to catalyze synthetic hydrazide compounds, enabling the bacteria to grow with them as a sole carbon source, but natural substrates have remained unknown. In this study, kinetic analyses of hydrazidase with parabens showed that the compounds can be substrates. Then, methylparaben induced gene expressions of the operon containing hydrazidase and ABC transporter, and the compound as a sole carbon source was able to grow the bacteria. Furthermore, homology search was carried out revealing that several actinomycetes possess hydrazidase homologs in the operon. Among those bacteria, an amidase from Pseudonocardia acaciae was subjected to a kinetic analysis and a structure determination revealing similar but not identical to those of hydrazidase. Since parabens are reported to exist in plants and soil, and several actinomycetes code the homologous operon, the enzymes with those operons may play a physiologically important role for bacterial survival with use of parabens.

Characterizing an amidase and its operon from actinomycete bacteria responsible for paraben catabolism.,Takenoya M, Hiratsuka Y, Shimamura K, Ito S, Sasaki Y, Yajima S Biosci Biotechnol Biochem. 2024 Aug 26;88(9):1047-1054. doi: 10.1093/bbb/zbae083. PMID:38886122<ref>PMID:38886122</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8xac" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudonocardia acaciae]]
[[Category: Takenoya M]]
[[Category: Yajima S]]