View source for 8xiu


==Cryo-EM structure of a frog VMAT2 in an apo conformation==
<StructureSection load='8xiu' size='340' side='right'caption='[[8xiu]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8xiu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XIU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xiu OCA], [https://pdbe.org/8xiu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xiu RCSB], [https://www.ebi.ac.uk/pdbsum/8xiu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xiu ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Vesicular monoamine transporter 2 (VMAT2) belongs to the major facilitator superfamily (MFS), and mediates cytoplasmic monoamine packaging into presynaptic vesicles. Here, we present two cryo-EM structures of VMAT2, with a frog VMAT2 adopting a canonical MFS fold and an engineered sheep VMAT2 adopting a non-canonical fold. Both VMAT2 proteins mediate uptake of a selective fluorescent VMAT2 substrate into cells. Molecular docking, substrate binding and transport analysis reveal potential substrate binding mechanism in VMAT2. Meanwhile, caution is advised when interpreting engineered membrane protein structures.

Engineering of a mammalian VMAT2 for cryo-EM analysis results in non-canonical protein folding.,Lyu Y, Fu C, Ma H, Su Z, Sun Z, Zhou X Nat Commun. 2024 Aug 2;15(1):6511. doi: 10.1038/s41467-024-50934-5. PMID:39095428<ref>PMID:39095428</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8xiu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Xenopus laevis]]
[[Category: Fu C]]
[[Category: Lyu Y]]
[[Category: Ma H]]
[[Category: Su Z]]
[[Category: Sun Z]]
[[Category: Zhou X]]