View source for 8xoc


==beta-1,4-galacosyltransferase==
<StructureSection load='8xoc' size='340' side='right'caption='[[8xoc]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8xoc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aggregatibacter_actinomycetemcomitans_NUM4039 Aggregatibacter actinomycetemcomitans NUM4039]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XOC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDU:GALACTOSE-URIDINE-5-DIPHOSPHATE'>GDU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xoc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xoc OCA], [https://pdbe.org/8xoc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xoc RCSB], [https://www.ebi.ac.uk/pdbsum/8xoc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xoc ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lacto-N-neotetraose (LNnT), a representative oligosaccharide found in human milk, has been previously examined for its beneficial traits. However, the LNnT titer is limited by the efficient glycosyltransferase pathway, particularly with respect to the catalysis of rate-limiting steps. As data on the crystal structure of the key enzyme required for synthesizing LNnT are lacking, the synthesis of LNnT remains an uncertainty. Here, for the first time we report the three-dimensional structure of a bacterial beta-1,4-galactosyltransferase, Aabeta4GalT, and analyze the critical role played by residues in its catalytic efficacy. Guided by structural insights, we engineered this enzyme to enhance its catalytic efficiency using structure-guided tunnel engineering. The mutant enzyme L5 (K155M/H156D/F157W/K185M/Q216V) so produced, showed a 50-fold enhancement in catalytic activity. Crystal structure analysis revealed that the mechanism underlying the improvement in activity was of the swing door type. The closed conformation formed by dense hydrophobic packing with Q216V-K155M widened and permitted substrate entry. Our results show that altering the tunnel conformation helped appropriately accommodate the substrate for catalysis and provide a structural basis for the modification of other glycosyltransferases.

Crystal structure and structure-guided tunnel engineering in a bacterial beta-1,4-galactosyltransferase.,Luo G, Huang Z, Zhu Y, Chen J, Hou X, Ni D, Xu W, Zhang W, Rao Y, Mu W Int J Biol Macromol. 2024 Nov;279(Pt 4):135374. doi: , 10.1016/j.ijbiomac.2024.135374. Epub 2024 Sep 10. PMID:39265897<ref>PMID:39265897</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8xoc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aggregatibacter actinomycetemcomitans NUM4039]]
[[Category: Large Structures]]
[[Category: Chen J]]
[[Category: Hou X]]
[[Category: Huang Z]]
[[Category: Luo G]]
[[Category: Mu W]]
[[Category: Ni D]]
[[Category: Rao Y]]
[[Category: Xu W]]
[[Category: Zhang W]]
[[Category: Zhu Y]]