View source for 8xqx


==Cryo-EM structure of the Ycf2-FtsHi motor complex from chlamydomonas reinhardtii in apo state==
<StructureSection load='8xqx' size='340' side='right'caption='[[8xqx]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8xqx]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XQX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xqx OCA], [https://pdbe.org/8xqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xqx RCSB], [https://www.ebi.ac.uk/pdbsum/8xqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xqx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2K3DLI2_CHLRE A0A2K3DLI2_CHLRE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The protein import motor in chloroplasts plays a pivotal role in their biogenesis and homeostasis by driving the translocation of preproteins into chloroplasts. While the Ycf2-FtsHi complex serves as the import motor in land plants, its evolutionary conservation, specialization, and mechanisms across photosynthetic organisms are largely unexplored. Here, we isolated and determined the cryogenic electron microscopy (cryo-EM) structures of the native Ycf2-FtsHi complex from Chlamydomonas reinhardtii, uncovering a complex composed of up to 19 subunits, including multiple green-algae-specific components. The heterohexameric AAA+ ATPase motor module is tilted, potentially facilitating preprotein handover from the translocon at the inner chloroplast membrane (TIC) complex. Preprotein interacts with Ycf2-FtsHi and enhances its ATPase activity in vitro. Integrating Ycf2-FtsHi and translocon at the outer chloroplast membrane (TOC)-TIC supercomplex structures reveals insights into their physical and functional interplay during preprotein translocation. By comparing these findings with those from land plants, our study establishes a structural foundation for understanding the assembly, function, evolutionary conservation, and diversity of chloroplast protein import motors.

Conservation and specialization of the Ycf2-FtsHi chloroplast protein import motor in green algae.,Liang K, Zhan X, Li Y, Yang Y, Xie Y, Jin Z, Xu X, Zhang W, Lu Y, Zhang S, Zou Y, Feng S, Wu J, Yan Z Cell. 2024 Aug 26:S0092-8674(24)00893-6. doi: 10.1016/j.cell.2024.08.002. PMID:39197449<ref>PMID:39197449</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8xqx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Chlamydomonas reinhardtii]]
[[Category: Large Structures]]
[[Category: Liang K]]
[[Category: Wu J]]
[[Category: Yan Z]]
[[Category: Zhan X]]