View source for 8xx5


==ASFV RNAP M1249L C-tail occupied complex1 (MCOC1)==
<StructureSection load='8xx5' size='340' side='right'caption='[[8xx5]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8xx5]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/African_swine_fever_virus African swine fever virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XX5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xx5 OCA], [https://pdbe.org/8xx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xx5 RCSB], [https://www.ebi.ac.uk/pdbsum/8xx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xx5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2X0RUE7_ASF A0A2X0RUE7_ASF] Component of the DNA-directed RNA polymerase (RNAP) that catalyzes the transcription in the cytoplasm of viral DNA into RNA using the four ribonucleoside triphosphates as substrates.[ARBA:ARBA00029376]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
African swine fever virus is highly contagious and causes a fatal infectious disease in pigs, resulting in a significant global impact on pork supply. The African swine fever virus RNA polymerase serves as a crucial multifunctional protein complex responsible for genome transcription and regulation. Therefore, it is essential to investigate its structural and functional characteristics for the prevention and control of African swine fever. Here, we determine the structures of endogenous African swine fever virus RNA polymerase in both nucleic acid-free and elongation states. The African swine fever virus RNA polymerase shares similarities with the core of typical RNA polymerases, but possesses a distinct subunit M1249L. Notably, the dynamic binding mode of M1249L with RNA polymerase, along with the C-terminal tail insertion of M1249L in the active center of DNA-RNA scaffold binding, suggests the potential of M1249L to regulate RNA polymerase activity within cells. These results are important for understanding the transcription cycle of the African swine fever virus and for developing antiviral strategies.

Structural basis of RNA polymerase complexes in African swine fever virus.,Zhu G, Xi F, Zeng W, Zhao Y, Cao W, Liu C, Yang F, Ru Y, Xiao S, Zhang S, Liu H, Tian H, Yang F, Lu B, Sun S, Song H, Sun B, Zhao X, Tang L, Li K, He J, Guo J, Zhu Y, Zhu Z, Sun F, Zheng H Nat Commun. 2025 Jan 8;16(1):501. doi: 10.1038/s41467-024-55683-z. PMID:39779680<ref>PMID:39779680</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8xx5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: African swine fever virus]]
[[Category: Large Structures]]
[[Category: Sun F]]
[[Category: Zheng HX]]
[[Category: Zhu GL]]
[[Category: Zhu Y]]
[[Category: Zhu ZX]]