View source for 8xzp


==Crystal structure of folE riboswitch with 8-CH3 Guanine==
<StructureSection load='8xzp' size='340' side='right'caption='[[8xzp]], [[Resolution|resolution]] 2.18&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8xzp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Unidentified_eubacterium_clone_A70 Unidentified eubacterium clone A70]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8XZP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8XZP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.18&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1LXN:2-azanyl-8-methyl-1,9-dihydropurin-6-one'>A1LXN</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8xzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8xzp OCA], [https://pdbe.org/8xzp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8xzp RCSB], [https://www.ebi.ac.uk/pdbsum/8xzp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8xzp ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Riboswitches are conserved regulatory RNA elements participating in various metabolic pathways. Recently, a novel RNA motif known as the folE RNA motif was discovered upstream of folE genes. It specifically senses tetrahydrofolate (THF) and is therefore termed THF-II riboswitch. To unravel the ligand recognition mechanism of this newly discovered riboswitch and decipher the underlying principles governing its tertiary folding, we determined both the free-form and bound-form THF-II riboswitch in the wild-type sequences. Combining structural information and isothermal titration calorimetry (ITC) binding assays on structure-based mutants, we successfully elucidated the significant long-range interactions governing the function of THF-II riboswitch and identified additional compounds, including alternative natural metabolites and potential lead compounds for drug discovery, that interact with THF-II riboswitch. Our structural research on the ligand recognition mechanism of the THF-II riboswitch not only paves the way for identification of compounds targeting riboswitches, but also facilitates the exploration of THF analogs in diverse biological contexts or for therapeutic applications.

Structure-based characterization and compound identification of the wild-type THF class-II riboswitch.,Li C, Xu X, Geng Z, Zheng L, Song Q, Shen X, Wu J, Zhao J, Li H, He M, Tai X, Zhang L, Ma J, Dong Y, Ren A Nucleic Acids Res. 2024 May 20:gkae377. doi: 10.1093/nar/gkae377. PMID:38769061<ref>PMID:38769061</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8xzp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Unidentified eubacterium clone A70]]
[[Category: Li CY]]
[[Category: Ren AM]]