View source for 8y2z


==MPXV mRNA cap N7 methyltransferase==
<StructureSection load='8y2z' size='340' side='right'caption='[[8y2z]], [[Resolution|resolution]] 3.94&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8y2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Monkeypox_virus Monkeypox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8Y2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8Y2Z FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.94&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8y2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8y2z OCA], [https://pdbe.org/8y2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8y2z RCSB], [https://www.ebi.ac.uk/pdbsum/8y2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8y2z ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MCEL_MONPV MCEL_MONPV] Catalytic subunit of the mRNA capping enzyme which catalyzes three enzymatic reactions: the 5' triphosphate end of the pre-mRNA is hydrolyzed to a diphosphate by RNA 5' triphosphatase; the diphosphate RNA end is capped with GMP by RNA guanylyltransferase and the GpppN cap is methylated by RNA (guanine-N7) methyltransferase. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the methyltransferase OPG102 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure). Also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I/OPG123 and the RAP94/OPG109 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94/OPG109, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA.[UniProtKB:P04298]
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</StructureSection>
[[Category: Large Structures]]
[[Category: Monkeypox virus]]
[[Category: Chen AK]]
[[Category: Li JX]]