View source for 8yag


==Cryo-electron microscopic structure of an amide hydrolase from Pseudoxanthomonas wuyuanensis==
<StructureSection load='8yag' size='340' side='right'caption='[[8yag]], [[Resolution|resolution]] 2.33&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8yag]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudoxanthomonas_wuyuanensis Pseudoxanthomonas wuyuanensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8YAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8YAG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.33&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8yag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8yag OCA], [https://pdbe.org/8yag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8yag RCSB], [https://www.ebi.ac.uk/pdbsum/8yag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8yag ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A286D6Z1_9GAMM A0A286D6Z1_9GAMM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ochratoxin A (OTA) contamination in various agro-products poses a serious threat to the global food safety and human health, leading to enormous economic losses. Enzyme-mediated OTA degradation is an appealing strategy, and the search for more efficient enzymes is a prerequisite for achieving this goal. Here, a novel amidohydrolase, termed PwADH, was demonstrated to exhibit 7.3-fold higher activity than that of the most efficient OTA-degrading ADH3 previously reported. Cryo-electron microscopy structure analysis indicated that additional hydrogen-bond interactions among OTA and the adjacent residue H163, the more compact substrate-binding pocket, and the wider entry to the substrate-access cavity might account for the more efficient OTA-degrading activity of PwADH compared with that of ADH3. We conducted a structure-guided rational design of PwADH and obtained an upgraded variant, G88D, whose OTA-degrading activity was elevated by 1.2-fold. In addition, PwADH and the upgraded G88D were successfully expressed in the industrial yeast Pichia pastoris, and their catalytic activities were compared to those of their counterparts produced in E. coli, revealing the feasibility of producing PwADH and its variants in industrial yeast strains. These results illustrate the structural basis of a novel, efficient OTA-degrading amidohydrolase and will be beneficial for the development of high-efficiency OTA-degrading approaches.

Functional characterization and structural basis of an efficient ochratoxin A-degrading amidohydrolase.,Hu Y, Dai L, Xu Y, Niu D, Yang X, Xie Z, Shen P, Li X, Li H, Zhang L, Min J, Guo RT, Chen CC Int J Biol Macromol. 2024 Oct;278(Pt 3):134831. doi: , 10.1016/j.ijbiomac.2024.134831. Epub 2024 Aug 18. PMID:39163957<ref>PMID:39163957</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8yag" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudoxanthomonas wuyuanensis]]
[[Category: Chen C-C]]
[[Category: Dai LH]]
[[Category: Guo R-T]]
[[Category: Hu YM]]
[[Category: Li H]]
[[Category: Li X]]
[[Category: Niu D]]
[[Category: Shen PP]]
[[Category: Xie ZZ]]
[[Category: Xu YH]]
[[Category: Yang XC]]