View source for 8ysr


==Crystal structure of the Deinococcus wulumuqiensis CD-NTase DwCdnB in complex with hexatungstotellurate(VI) TEW==
<StructureSection load='8ysr' size='340' side='right'caption='[[8ysr]], [[Resolution|resolution]] 2.04&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ysr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_wulumuqiensis Deinococcus wulumuqiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8YSR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8YSR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.04&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ysr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ysr OCA], [https://pdbe.org/8ysr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ysr RCSB], [https://www.ebi.ac.uk/pdbsum/8ysr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ysr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A345ILN6_9DEIO A0A345ILN6_9DEIO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Radiation-resistant bacteria are of great application potential in various fields, including bioindustry and bioremediation of radioactive waste. However, how radiation-resistant bacteria combat against invading phages is seldom addressed. Here, we present a series of crystal structures of a sensor and an effector of the cyclic oligonucleotide-based anti-phage signaling system (CBASS) from a radioresistant bacterium Deinococcus wulumuqiensis. We found that the sensor CD-NTase enzyme, DwCdnB, can bind all four ribonucleotides and synthesize a variety of cyclic di-nucleotides, including the novel second messenger 3'3'-cyclic di-CMP. Crystal structures of DwCdnB in complex with ATP and dATP provide structural explanations for specific recognition of ribonucleotides via metal coordination with ribose 2'-OH. Crystal structures of DwCdnB in complex with purine and/or pyrimidine nucleotides in the presence of Mg(2+) revealed similar binding modes; however, in the presence of Mn(2+), the UTP/CTP rotates and flips into the donor pocket and make extensive contacts with additional five residues, suggesting essential role of Mn(2+) for catalytic production of cyclic di-pyrimidines. Finally, structural analysis of the downstream effector DwCap5 further provides a structural explanation for its non-specific recognition of a broad range of cyclic di-nucleotides. In sum, this work provides key structural insights into the immune mechanisms of radioresistant bacteria.

Structural insights into signaling promiscuity of the CBASS anti-phage defense system from a radiation-resistant bacterium.,Yang CS, Shie MY, Huang SW, Wang YC, Hou MH, Chen CJ, Chen Y Int J Biol Macromol. 2025 Jan 4;295:139534. doi: 10.1016/j.ijbiomac.2025.139534. PMID:39761885<ref>PMID:39761885</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ysr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Deinococcus wulumuqiensis]]
[[Category: Large Structures]]
[[Category: Chen Y]]
[[Category: Hou M-H]]
[[Category: Wang Y-C]]
[[Category: Yang C-S]]