View source for 8ytw


==Kubu-PETase from Kutzneria buriramensis==
<StructureSection load='8ytw' size='340' side='right'caption='[[8ytw]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8ytw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kutzneria_buriramensis Kutzneria buriramensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8YTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8YTW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8ytw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8ytw OCA], [https://pdbe.org/8ytw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8ytw RCSB], [https://www.ebi.ac.uk/pdbsum/8ytw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8ytw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A3E0H050_9PSEU A0A3E0H050_9PSEU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Enzymes capable of breaking down polymers have been identified from natural sources and developed for industrial use in plastic recycling. However, there are many potential starting points for enzyme optimization that remain unexplored. We generated a landscape of 170 lineages of 1894 polyethylene terephthalate depolymerase (PETase) candidates and performed profiling using sampling approaches with features associated with PET-degrading capabilities. We identified three promising yet unexplored PETase lineages and two potent PETases, Mipa-P and Kubu-P. An engineered variant of Kubu-P outperformed benchmarks in terms of PET depolymerization in harsh environments, such as those with high substrate load and ethylene glycol as the solvent.

Landscape profiling of PET depolymerases using a natural sequence cluster framework.,Seo H, Hong H, Park J, Lee SH, Ki D, Ryu A, Sagong HY, Kim KJ Science. 2025 Jan 3;387(6729):eadp5637. doi: 10.1126/science.adp5637. Epub 2025 , Jan 3. PMID:39745946<ref>PMID:39745946</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8ytw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Kutzneria buriramensis]]
[[Category: Large Structures]]
[[Category: Hong H]]
[[Category: Kim K-J]]
[[Category: Park J]]
[[Category: Seo H]]