View source for 8yty


==The M12+P185V variant of Kubu-PETase from Kutzneria buriramensis==
<StructureSection load='8yty' size='340' side='right'caption='[[8yty]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8yty]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kutzneria_buriramensis Kutzneria buriramensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8YTY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8YTY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8yty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8yty OCA], [https://pdbe.org/8yty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8yty RCSB], [https://www.ebi.ac.uk/pdbsum/8yty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8yty ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A3E0H050_9PSEU A0A3E0H050_9PSEU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Enzymes capable of breaking down polymers have been identified from natural sources and developed for industrial use in plastic recycling. However, there are many potential starting points for enzyme optimization that remain unexplored. We generated a landscape of 170 lineages of 1894 polyethylene terephthalate depolymerase (PETase) candidates and performed profiling using sampling approaches with features associated with PET-degrading capabilities. We identified three promising yet unexplored PETase lineages and two potent PETases, Mipa-P and Kubu-P. An engineered variant of Kubu-P outperformed benchmarks in terms of PET depolymerization in harsh environments, such as those with high substrate load and ethylene glycol as the solvent.

Landscape profiling of PET depolymerases using a natural sequence cluster framework.,Seo H, Hong H, Park J, Lee SH, Ki D, Ryu A, Sagong HY, Kim KJ Science. 2025 Jan 3;387(6729):eadp5637. doi: 10.1126/science.adp5637. Epub 2025 , Jan 3. PMID:39745946<ref>PMID:39745946</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8yty" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Kutzneria buriramensis]]
[[Category: Large Structures]]
[[Category: Hong H]]
[[Category: Kim K-J]]
[[Category: Park J]]
[[Category: Seo H]]