View source for 8yz2


==Cryo-EM structure of a tri-heme cytochrome-associated RC-LH1 complex from a marine photoheterotrophic bacterium, purified with magnesium solutions==
<StructureSection load='8yz2' size='340' side='right'caption='[[8yz2]], [[Resolution|resolution]] 2.68&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8yz2]] is a 39 chain structure with sequence from [https://en.wikipedia.org/wiki/Dinoroseobacter_shibae_DFL_12_=_DSM_16493 Dinoroseobacter shibae DFL 12 = DSM 16493]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8YZ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8YZ2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.68&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1EFU:(4~{E},16~{E},26~{E})-2-methoxy-2,6,10,14,19,23,27,31-octamethyl-dotriaconta-4,6,8,10,12,14,16,18,20,22,26,30-dodecaen-3-one'>A1EFU</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=MW9:[(2~{R})-3-[[(2~{S})-2,3-bis(oxidanyl)propoxy]-oxidanyl-phosphoryl]oxy-2-[(~{Z})-octadec-9-enoyl]oxy-propyl]+octadecanoate'>MW9</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8yz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8yz2 OCA], [https://pdbe.org/8yz2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8yz2 RCSB], [https://www.ebi.ac.uk/pdbsum/8yz2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8yz2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A8LQ15_DINSH A8LQ15_DINSH] Antenna complexes are light-harvesting systems, which transfer the excitation energy to the reaction centers.[ARBA:ARBA00002455]
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== Publication Abstract from PubMed ==
The reaction center-light harvesting 1 (RC-LH1) complex converts solar energy into electrical energy, driving the initiation of photosynthesis. The authors present a cryo-electron microscopy structure of the RC-LH1 isolated from a marine photoheterotrophic bacterium Dinoroseobacter shibae. The RC comprises four subunits, including a three-heme cytochrome (Cyt) c protein, and is surrounded by a closed LH ring composed of 17 pairs of antenna subunits. Notably, a novel subunit with an N-terminal "helix-turn-helix" motif embedded in the gap between the RC and the LH ring is identified. The purified RC-LH1 complex exhibits high stability in solutions containing Mg(2+) or Ca(2+). The periplasmic Cyt c(2) is predicted to bind at the junction between the Cyt subunit and the membrane plane, enabling electron transfer from Cyt c(2) to the proximal heme of the tri-heme Cyt, and subsequently to the special pair of bacteriochlorophylls. These findings provide structural insights into the efficient energy and electron transfer processes within a distinct type of RC-LH1, and shed light on evolutionary adaptations of photosynthesis.

Cryo-EM Analysis of a Tri-Heme Cytochrome-Associated RC-LH1 Complex from the Marine Photoheterotrophic Bacterium Dinoroseobacter Shibae.,Wang W, Liu Y, Gu J, An S, Ma C, Gao H, Jiao N, Shen JR, Beatty JT, Koblizek M, Zhang X, Zheng Q, Chen JH Adv Sci (Weinh). 2025 Mar 20:e2413456. doi: 10.1002/advs.202413456. PMID:40112203<ref>PMID:40112203</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 8yz2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Dinoroseobacter shibae DFL 12 = DSM 16493]]
[[Category: Large Structures]]
[[Category: Chen JH]]
[[Category: Zhang X]]
[[Category: Zheng Q]]