View source for 8zaq


==ExoC110T class 2 channelrhodopsin==
<StructureSection load='8zaq' size='340' side='right'caption='[[8zaq]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8zaq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Hyphochytrium_catenoides Hyphochytrium catenoides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ZAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ZAQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8zaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8zaq OCA], [https://pdbe.org/8zaq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8zaq RCSB], [https://www.ebi.ac.uk/pdbsum/8zaq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8zaq ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Channelrhodopsins are popular optogenetic tools in neuroscience, but remain poorly understood mechanistically. Here we report the cryo-EM structures of channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii and H. catenoides kalium channelrhodopsin (KCR1). We show that ChR2 recruits an endogenous N-retinylidene-PE-like molecule to a previously unidentified lateral retinal binding pocket, exhibiting a reduced light response in HEK293 cells. In contrast, H. catenoides kalium channelrhodopsin (KCR1) binds an endogenous retinal in its canonical retinal binding pocket under identical condition. However, exogenous ATR reduces the photocurrent magnitude of wild type KCR1 and also inhibits its leaky mutant C110T. Our results uncover diverse retinal chromophores with distinct binding patterns for channelrhodopsins in mammalian cells, which may further inspire next generation optogenetics for complex tasks such as cell fate control.

Channelrhodopsins with distinct chromophores and binding patterns.,Shan Y, Zhao L, Chen M, Li X, Zhang M, Pei D Nat Commun. 2024 Aug 24;15(1):7292. doi: 10.1038/s41467-024-51811-x. PMID:39181878<ref>PMID:39181878</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Hyphochytrium catenoides]]
[[Category: Large Structures]]
[[Category: Zhang MF]]