View source for 8zex


==Biosensor HaloKbp1a==
<StructureSection load='8zex' size='340' side='right'caption='[[8zex]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8zex]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ZEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ZEX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8zex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8zex OCA], [https://pdbe.org/8zex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8zex RCSB], [https://www.ebi.ac.uk/pdbsum/8zex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8zex ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Potassium ion (K(+)) is the most abundant metal ion in cells and plays an indispensable role in practically all biological systems. Although there have been reports of both synthetic and genetically encoded fluorescent K(+) indicators, there remains a need for an indicator that is genetically targetable, has high specificity for K(+) versus Na(+), and has a high fluorescent response in the red to far-red wavelength range. Here, we introduce a series of chemigenetic K(+) indicators, designated as the HaloKbp1 series, based on the bacterial K(+)-binding protein (Kbp) inserted into HaloTag7 self-labeled with environmentally sensitive rhodamine derivatives. This series of high-performance indicators features high brightness in the red to far-red region, large intensiometric fluorescence changes, and a range of K(d) values. We demonstrate that they are suitable for the detection of physiologically relevant K(+) concentration changes such as those that result from the Ca(2+)-dependent activation of the BK potassium channel.

High-Performance Chemigenetic Potassium Ion Indicator.,Cheng D, Ouyang Z, He X, Nasu Y, Wen Y, Terai T, Campbell RE J Am Chem Soc. 2024 Nov 27. doi: 10.1021/jacs.4c10917. PMID:39601449<ref>PMID:39601449</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8zex" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Campbell RE]]
[[Category: Wen Y]]