View source for 8zvk


==Crystal structure of the GH5 domain from a processive endoglucanase of Acetivibrio alkalicellulosi==
<StructureSection load='8zvk' size='340' side='right'caption='[[8zvk]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8zvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio Acetivibrio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ZVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ZVK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8zvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8zvk OCA], [https://pdbe.org/8zvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8zvk RCSB], [https://www.ebi.ac.uk/pdbsum/8zvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8zvk ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cellulose, a linear glucan linked by beta-1,4 glycosidic bonds, is the most abundant renewable polysaccharide on earth. Complete enzymatic hydrolysis of cellulose liberates the readily metabolizable glucose that could be further converted to valuable biocommodities, and essential to this process are cellulases that hydrolyze the beta-1,4 glycosidic bonds. Cellulases are among the most intensively studied and best understood enzymes, and many key residues have been uncovered and interrogated with respect to their functions in catalysis and/or substrate binding. However, it remains to be explored whether additional residues, especially in many poorly characterized cellulases such as processive endoglucanases, might also be functionally important. Here, we investigated a processive endoglucanase from an alkaliphilic bacterium Acetivibrio alkalicellulosi AaCel5A that consists of a glycohydrolase family 5 (GH5) domain and two tandem carbohydrate-binding module family 6 (CBM6) domains. Via structure-guided engineering, we uncovered the functional importance of a previously underexplored but relatively conserved histidine (histidine70 or His(70)). His(70) itself appears to be largely dispensable for hydrolyzing beta-1,4 glycosidic bonds, but it is important for efficient hydrolysis of short cellodextrins such as cellotriose, cellotetraose, and cellopentaose, likely through its ability to coordinate substrate binding. Our work thus provides important mechanistic insights into how processive endoglucanases may act on short cellodextrins.

His(70) of Acetivibrio alkalicellulosi Cel5A is important for efficient hydrolysis of short cellodextrins.,Jia M, Chen Y, Wang J, Wang J, Ma Y, Wang Y, Ma Q, Zhang Y, Liu W, Liu K AMB Express. 2025 Mar 20;15(1):53. doi: 10.1186/s13568-025-01858-w. PMID:40111668<ref>PMID:40111668</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8zvk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Acetivibrio]]
[[Category: Large Structures]]
[[Category: Chen YY]]
[[Category: Jia MX]]
[[Category: Liu KQ]]
[[Category: Liu WD]]
[[Category: Ma Q]]
[[Category: Ma YH]]
[[Category: Wang JH]]
[[Category: Zhang YH]]