View source for 9ard


==Structure of Pycsar EcPycC cyclase immunoglobulin-like AGS-C domain==
<StructureSection load='9ard' size='340' side='right'caption='[[9ard]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9ard]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9ARD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9ARD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ard FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ard OCA], [https://pdbe.org/9ard PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ard RCSB], [https://www.ebi.ac.uk/pdbsum/9ard PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ard ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PYCC1_ECOLX PYCC1_ECOLX] Pycsar (pyrimidine cyclase system for antiphage resistance) provides immunity against bacteriophage. The pyrimidine cyclase (PycC) synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signal activates the adjacent effector, leading to bacterial cell death and abortive phage infection. A clade E Pycsar system.<ref>PMID:34644530</ref>   The pyrimidine cyclase gene of a two-gene Pycsar system, generates cyclic CMP (cCMP) from CTP in response to bacteriophage infection. Has little to no activity on ATP, GTP or UTP. Expression of this and adjacent effector EcPycTM (AC P0DV25) confers resistance to bacteriophage P1 and T5; expression of this gene alone does not confer resistance. When cells expressing the Pycsar system are infected by phage T5 at low multiplicity of infection (0.2 MOI) the culture survives, at 2.0 MOI bacteria enter growth arrest. The same cells enter growth arrest after exposure to 250 uM cCMP but not cUMP; thus the effector protein responds only to the cNMP produced by its cognate NTP cyclase. Some of the cells treated with cCMP have abnormal membrane protrusions.<ref>PMID:34644530</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Kranzusch PJ]]
[[Category: Richmond-Buccola D]]