View source for 9arl


==CryoEM structure of BoNT-NTNH-OrfX2 complex from Clostridium botulinum strain A1-ST7B, major class==
<StructureSection load='9arl' size='340' side='right'caption='[[9arl]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9arl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum_A Clostridium botulinum A] and [https://en.wikipedia.org/wiki/Clostridium_botulinum_A_str._Hall Clostridium botulinum A str. Hall]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9ARL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9ARL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9arl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9arl OCA], [https://pdbe.org/9arl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9arl RCSB], [https://www.ebi.ac.uk/pdbsum/9arl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9arl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
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</StructureSection>
[[Category: Clostridium botulinum A]]
[[Category: Clostridium botulinum A str. Hall]]
[[Category: Large Structures]]
[[Category: Gao L]]