View source for 9b41


==Pseudomonas phage Pa193 Neck (portal and head-to-tail proteins)==
<StructureSection load='9b41' size='340' side='right'caption='[[9b41]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9b41]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_Pa193 Pseudomonas virus Pa193]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9B41 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9B41 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9b41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9b41 OCA], [https://pdbe.org/9b41 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9b41 RCSB], [https://www.ebi.ac.uk/pdbsum/9b41 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9b41 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A5P1KVD8_9CAUD A0A5P1KVD8_9CAUD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The World Health Organization has designated Pseudomonas aeruginosa as a critical pathogen for the development of new antimicrobials. Bacterial viruses, or bacteriophages, have been used in various clinical settings, commonly called phage therapy, to address this growing public health crisis. Here, we describe a high-resolution structural atlas of a therapeutic, contractile-tailed Pseudomonas phage, Pa193. We used bioinformatics, proteomics, and cryogenic electron microscopy single particle analysis to identify, annotate, and build atomic models for 21 distinct structural polypeptide chains forming the icosahedral capsid, neck, contractile tail, and baseplate. We identified a putative scaffolding protein stabilizing the interior of the capsid 5-fold vertex. We also visualized a large portion of Pa193 ~ 500 A long tail fibers and resolved the interface between the baseplate and tail fibers. The work presented here provides a framework to support a better understanding of phages as biomedicines for phage therapy and inform engineering opportunities.

Cryo-EM analysis of Pseudomonas phage Pa193 structural components.,Iglesias SM, Hou CD, Reid J, Schauer E, Geier R, Soriaga A, Sim L, Gao L, Whitelegge J, Kyme P, Birx D, Lemire S, Cingolani G Commun Biol. 2024 Oct 6;7(1):1275. doi: 10.1038/s42003-024-06985-x. PMID:39370451<ref>PMID:39370451</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9b41" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas virus Pa193]]
[[Category: Cingolani G]]
[[Category: Iglesias SM]]